To optimize indirect regeneration (IR) and direct regeneration (DR) in Rosa hybrida cv. Apollo different explant types and different concentrations of plant growth regulators were investigated. Among the different auxins studied and over all explant types, 10 µM 2,4-dichlorophenoxyacetic acid (2,4-D) promoted the highest frequency of callus production for IR. The highest frequency of regeneration (60.8 %) was obtained when calli were transferred to Murashige and Skoog medium supplemented with 2.5 µM thidiazuron (TDZ) and 2 µM gibberellic acid. The highest frequency of regeneration (80.2 %) for DR was obtained from leaves cultured on the medium containing 10 µM TDZ. The efficiency of IR and DR were compared in four different rose cultivars including ‘Apollo’, ‘Black Baccara’, ‘Maroussia’ and ‘Amanda’. The frequency of regeneration in all four cultivars was significantly higher in DR compared to IR. Also shoots regenerated by DR appeared earlier than the shoots regenerated by IR. The results of flow cytometry showed that the shoots derived from IR to DR were tetraploid like the original cultivars.
Financed by the National Centre for Research and Development under grant No. SP/I/1/77065/10 by the strategic scientific research and experimental development program:
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