This paper presents analytical derivations of the pressure distribution in straight and uniform rectangular microchannels in the slip flow regime and new experimental data in those channels. The flow is to be steady state, two-dimensional, isothermal, and to have negligible transverse velocities with a first order slip boundary condition. The measured pressure distributions of airflows are compared with newly derived analytical results. There is close agreement between the measurements and calculation by the slip flow formula. The dimensionless location of the maximum deviation from the linear pressure distribution is found analytically and compared with the measurements. This dimensionless location of the maximum deviation increases with the increasing pressure ratios in the slip flow regime. The effect of several parameters such as the channel aspect ratio and the Knudsen number on the locations of maximum deviation from linearity are investigated. The nonlinearity of the pressure distribution is also discussed.