The feasibility of single molecule elastic scattering analysis with the X-ray free electron laser (XFEL) sources in operation and under construction around the world was investigated for various biological and synthetic materials (pepsin, polyethylene, poly(4,4′-oxydiphenylene pyromellitimide), and ferric oxide). It was found that existing XFEL facilities provide coherent pulse X-ray beams with the required energies (8.3–12.4 keV), but their fluxes are too low for single molecule elastic scattering experiment to determine the three-dimensional structures of such molecules. For single molecule scattering, the XFEL facilities need to improve their beam flux density to 2×1015 to 7×1018 photons pulse−1 μm−2, depending on the beam energy. However, the existing XFEL facilities’ sources were found to enable the elastic scattering analysis of pepsin and synthetic polymers with sample sizes of 1–160 μm, as well as of ferric oxide with sample sizes of ≥80 nm. These criteria for the sample size can be extended to other soft (biological, organic, and polymer molecules) and hard (molecules containing heavy metals) materials. In addition, the inelastic scattering, absorption, and radiation damage characteristics of the chosen materials when exposed to the XFEL sources were examined.