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A modified Agrobacterium-mediated transformation protocol has been successfully used for transient expression of the intrinsically fluorescent proteins and their fusion proteins in onion epidermis. The mean of the transformed cells rate per peel is about 10.5±0.9%, while that of the particle bombardment method is at the range 2.0±0.4%. To compare with the prevailing method of micro-projectile bombardment,...
The long-term trends of mean monthly nitrate concentrations in stream and drainage runoff were evaluated in the experimental microbasin Rybárik (0.119 km2) at the Institute of Hydrology, Slovak Academy of Sciences, during the period 1987–2005. The results of analyses indicate a decreasing trend of nitrate concentration after the year 1989, but with relatively high losses in some years and relatively...
Two-component signal transduction system (TCS) is a common mechanism of signal transduction and exists in almost all bacterial species. The putative two-component genes SCO6421 (ecrE1) and SCO6422 (ecrE2) in Streptomyces coelicolor, which located in the vicinity of the red locus, could modulate the antibiotic undecylprodigiosin (Red) biosynthesis. In this study, the mutants of M145E1 (ecrE1 knock-out)...
The aim of this research is to determine the probability of anterior and posterior presentation during the second half of gestation and at delivery among albino mice. Each day from the 10th to the 21st day of gestation, foetal presentation and lie were determined in uteri of six pregnant females. The chi-square test did not show any statistically significant difference in the incidence of anterior...
Experimental studies of regeneration and repair processes of schistomelic walking legs and pedipalps in the early postembryonal stages of Tegenaria atrica were carried out in two parts. In the first part changes in the structure of appendages were produced by exposing the developing embryos to alternating temperatures (14 and 32°C, changing every 12 hours) as teratogenic factor. In the second, principal,...
Studies indicate that leptin is involved in not only energy expenditure and food intake, but also in protection against apoptosis, in inflammation and in stimulation of proliferation in many cell types. However, leptin treatment increases the oxidative stress in many cell culture studies. This contradiction evoked a question of whether leptin acts as an oxidant or antioxidant on glial cells. We investigated...
It was shown that compensatory base changes (CBCs) in internal transcribed spacer 2 (ITS2) sequence-structure alignments can be used for distinguishing species. Using the ITS2 Database in combination with 4SALE — a tool for synchronous RNA sequence and secondary structure alignment and editing — in this study we present an in-depth CBC analysis for placozoan ITS2 sequences and their respective secondary...
Genetic variation in six natural populations of Scots pine (Pinus sylvestris L.) was determined with isoenzyme analyses. For this purpose, haploid female gametophytes of seeds and horizontal starch gel electrophoresis technique were used. A total of 17 loci and 58 alleles were observed in studying 10 enzyme systems. The average proportion of polymorphic loci for populations ranged from 58.8% to 70...
Nine vegetation types were distinguished using cluster analysis within Molinion meadows in Slovakia. Vegetation of cluster 1 occurs on most acidic soils and is characterized by the occurrence of species of the Caricion fuscae alliance and of the Nardus grasslands. Vegetation of cluster 2 is also found on rather acidic soils but in contrast to cluster 1 vegetation it contains species of base-rich sites,...
Total lipid and the fatty acid compositions of phospholipid and triacylglycerol fractions, prepared from eggs, 3rd instars of larvae, pupae, male and female adults of Lertha sheppardi, were analyzed by gas chromatography and gas chromatography-mass spectrometry. The effect of diet (adults’ nutrition) on fatty acid composition of L. sheppardi adults was also investigated. Total lipid of L. sheppardi...
Molecular weights of the skeletal muscle myosin, actin, troponin C and tropomyosin were compared in two frog species, Hyla japonica and Xenopus tropicalis, by SDS-PAGE and Western blot. Polyclonal antibody was produced using H. japonica skeletal muscle as the antigen. Polyclonal antibodies to nematode (Caenorhabditis elegans), mold slime (Physarum polycephalum), crab (Pagurus japonicus) and chicken...
The genus Nepalomyia is the largest genus in the subfamily Peloropeodinae, with 47 described species. In the present paper the Nepalomyia pallipilosa species group is established for the following three described species and seven new species: Nepalomyia biseta sp. n., N. emeiensis sp. n., N. guangxiensis Zhang et Yang, N. liui sp. n., N. pallipilosa (Yang et Saigusa), N. ruiliensis Wang et Yang,...
Morphological and micromorphological characters of the nutlets of ten Onosma species in Turkey were reported. Studied fruit surfaces show some variations. Based on the structure and patterns of epidermis cells of the nutlets, two principal types can be distinguished. In Type 1, the epidermal cells of the nutlet surface are formed in a reticulate pattern with varied sizes and shapes of the meshes....
Adventitious organogenetic structures were produced in vitro using cotyledon explants excised from 14 day-old pepper seedlings. The best response was observed on MS medium containing 5.7 μM indole-3-acetic acid and 8.8 μM 6-benzylaminopurine. However, when transferred onto elongation medium (MS + 2.8 μM gibberellic acid), these structures frequently developed into leaf-like features rather than into...
In the period 1993–2006, during investigation of reproduction biology of the bearded tit, 106 deserted nests of the species were collected in Slovakia, Austria and Italy and their arthropod fauna was analyzed. Occasionally introduced individuals of the pseudoscorpion Lamprochernes nodosus, a frequent species in Central Europe, were recorded in the nests. Altogether 984 individuals and 33 species of...
The paper includes data on rare occurring pseudofilamentous freshwater red alga, Chroodactylon ornatum (C. Agardh) Basson, in Poland and Slovakia, Central Europe. The species is classified as widespread in the whole world in littoral of stagnant waters or in rivers but rarely noted. Original microphotographs and drawings of Ch. ornatum are presented.
A new rare cold-inducible (RCI) gene designated Cbrci35 was cloned from Capsella bursa-pastoris, an edible wild herb, using the rapid amplification of cDNA ends (RACE) method. The full-length cDNA of Cbrci35 (Database Accession No.: AY566573) was 1300 bp and contained a 978 bp ORF encoding a precursor of 326 amino acid residues with a 23 amino acids signal peptide. The predicted Cbrci35 protein contained...
In addition to antibiotic properties, medicinal plants are important sources of chemicals with potential application as pesticides. The present study deals with antitermitic potential of seed extracts of Withania somnifera (Indian ginseng), Croton tiglium (jamalgoota) and Hygrophila auriculata (talimkhana). The seed extracts caused changes in tunneling behaviour, number of bacterial colonies in hindgut...
We examined the microclimate of different grasslands and the relationships between the seasonal changes in the microclimatic conditions and the structural characteristics of orthopteran assemblages in the most typical grassland types of the plain, hilly and submontaneous territories of Central Europe in 2000–2005 years. According to our results: (i) The seasonal changes of humidity were characteristic...
The morphology of the spermatheca and eggs of Odontotarsus purpureolineatus were studied by optical microscopy and scanning electron microscopy. The spherical eggs were about 1.35 mm long and 1.09 mm wide. The egg batches generally consist of 13–14 eggs. The egg surface is covered by polygonal (hexagonal and pentagonal shapes prevail) ridges and tiny chorionic tubercles. There were 8–10 aero-micropylar...
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