Cell Biology and Toxicology

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International Congress on Hepatocytes, Abstracts

Cell Biology and Toxicology > 1997 > 13 > 1 > 1-65

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In vitro effects of Celiptium and MR 14504 on mature rat Leydig cell testosterone production

H. Al Kadri, J.C. Lancelot, M.A. Drosdowsky, A. Al Quirbi, more

Cell Biology and Toxicology > 1997 > 13 > 2 > 83-94

Percoll-purified mature rat Leydig cells have been used to evaluate the testicular toxicity of two highly potent intercalating agents (Celiptium and MR 14505). Testosterone secretion in the absence and in the presence of human chorionic gonadotropin (hCG) was measured to assess Leydig cell function. Celiptium and MR 14504 induce time- and dose-related inhibitory effects on the production of testosterone...

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Furaltadone cytotoxicity on three cell lines in the presence or absence of DMSO: Comparison with furazolidone

A. Stammati, F. Zampaglioni, F. Zucco

Cell Biology and Toxicology > 1997 > 13 > 2 > 125-130

Nitrofuran drugs have been studied on cellular systems in order to develop in vitro tests for safety assessment of food contaminants. In the present study we have tested furaltadone on three cell lines (HEp-2, Caco-2 and V79), using the same toxicity endpoints as in a previous study with furazolidone, namely cell viability and growth, colony-forming ability, LDH release, and O2 consumption. One of...

article

Comparison of amitriptyline metabolism in hepatocytes from streptozocin-induced diabetic rats and from non-diabetic rats

F. Coudoré, A. Besson, R. Shrivastava, A. Chevalier, more

Cell Biology and Toxicology > 1997 > 13 > 2 > 131-137

Biotransformation of amitriptyline (AMI) was studied at different intervals in freshly isolated hepatocytes from healthy or streptozocin-induced diabetic rats in order to investigate the influence of the diabetic state. Levels of free and conjugated AMI, demethylated and hydroxylated metabolites, were assessed by HPLC analysis. In hepatocytes isolated from diabetic rats, AMI was less completely metabolized...

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Comparative evaluation of the antiproliferative effect of cyclosporin A and γ-interferon on normal and HPV-transformed keratinocytes by cell counting, MTT assay and tritiated thymidine incorporation

A.V. Marionnet, G. Lizard, Y. Chardonnet, D. Schmitt

Cell Biology and Toxicology > 1997 > 13 > 2 > 115-123

We compared three techniques, the MTT tetrazolium assay, cell counting, and tritiated thymidine ([3H]TdR) incorporation assay to measure the antiproliferative effect of cyclosporin A (CsA) and interferon-γ (IFN-γ) on normal human skin keratinocyte cultures (NHK) used at the second passage and human papillomavirus type 16- and 18-transformed cell lines (EK16 and EK18) exposed continuously to the drugs...

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Limitations of alginate gels as a culture model for the study of the effects of UVA radiation on human dermal fibroblasts

L. Pascual Le Tallec, C. Korwin-Zmijowska, M. Adolphe

Cell Biology and Toxicology > 1997 > 13 > 2 > 95-102

Several studies were undertaken to develop three-dimensional (3-D) cell culture models that allow conditions closer to the in vivo situation. To this end, alginate gels were tested as a 3-D cell culture model that might be useful in the study of the effects of UVA on human dermal fibroblasts. Cell culture in alginate gels and the irradiation conditions were optimized. Results showed that optimized...

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Establishment and characterization of an abrin-resistant cell line

J.-L. Ko, J.-Y. Lin

Cell Biology and Toxicology > 1997 > 13 > 2 > 75-81

An abrin-resistant cell line, CHOR 3-4, was isolated from CHOK1 cells which were resistant to a high concentration of abrin (160 ng/ml), and had a 1000-fold higher resistance to abrin that that of CHOK1 cells. CHOR 3-4 cells were about 25-fold more resistant than CHOK1 cells to the N-glycosidase activity of abrin, which was measured by hydrolyzing the N-glycosidic bond of adenine 4324 nucleotide from...

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Culture and characterization of juvenile rabbit tenocytes

K. Bernard-Beaubois, C. Hecquet, O. Houcine, G. Hayem, more

Cell Biology and Toxicology > 1997 > 13 > 2 > 103-113

The culture of rabbit tenocytes could be a useful model in the study of the physiopathology and pharmacotoxicology of tendons. This work was undertaken to examine the in vitro behavior of tenocytes from juvenile rabbit Achilles tendons. We report observations of the morphological and biological characteristics of primary culture and subsequent passages of rabbit tendon cells cultured in monolayer...

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L-Oxothiazolidine 4-carboxylate pretreatment of isolated human peripheral blood lymphocytes reduces sulfur mustard cytotoxicity

C.L. Gross, K.C. Giles, W.J. Smith

Cell Biology and Toxicology > 1997 > 13 > 3 > 167-173

Sulfur mustard (HD, mustard gas) is a vesicant chemical warfare agent for which there is no specific medical countermeasure. A potential approach to combating the debilitating effects of this agent is the use of compounds that can react with this material before it interacts with critical macro-molecules. Glutathione (GSH), a tripeptide that exists in high concentrations in cells, reacts with HD and...

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Synthetic hispidin, a PKC inhibitor, is more cytotoxic toward cancer cells than normal cells in vitro

C. Gonindard, C. Bergonzi, C. Denier, C. Sergheraert, more

Cell Biology and Toxicology > 1997 > 13 > 3 > 141-153

The trypanocidal activity of naturally occurring 6-(3,4-dihydroxystyryl)-4-hydroxy-2-pyrone (hispidin) prompted us to examine its cytotoxic activity toward normal and cancerous cells in culture. Hispidin synthesized in our laboratory to a high degree of purity (checked by 1H and 13C NMR spectroscopy) was shown to be cytotoxic (between 10-3 mol/L and 10-7 mol/L) toward normal human MRC-5 fibroblasts,...

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In vitro modulation of the P450 activities of hamster and human lung slices

P.H.M. Hoet, M. Demedts, B. Nemery

Cell Biology and Toxicology > 1997 > 13 > 3 > 185-192

The respiratory tract is a portal of entry for many environmental chemicals. The respiratory tract plays an important role in the detoxification or metabolic activation of these chemicals, e.g., via cytochrome P450 enzymes. Alterations in the capabilities of these enzymes to metabolize inhaled compounds can, therefore, affect the toxicity of the chemicals. The pulmonary cytochrome P450 activity has...

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Structure-activity relationship in the induction of chromosomal aberrations and spindle disturbances in Chinese hamster epithelial liver cells by regioisomeric phenanthrene quinones

G. Turchi, H.R. Glatt, A. Seidel, A. Puliti, more

Cell Biology and Toxicology > 1997 > 13 > 3 > 155-165

Four regioisomeric phenanthrene (PH) quinones (Q) were investigated for their ability to induce chromosomal damage and spindle disturbances. PH 1,4-Q and PH 1,2-Q induced structural as well as numerical chromosomal aberrations, whereas the isomers PH 9,10-Q and PH 3,4-Q were virtually inactive in this respect. However, all four compounds enhanced the frequency of c-mitoses.

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In vitro toxicity induced by deoxynivalenol (DON) on human and rat granulomonocytic progenitors

S. Lautraite, D. Parent-Massin, B. Rio, H. Hoellinger

Cell Biology and Toxicology > 1997 > 13 > 3 > 175-183

Deoxynivalenol (DON) is a trichothecene mycotoxin produced by various species of fungi. Trichothecenes are known as major contaminants of cereals and cereal-containing foods. DON has been detected in agricultural products worldwide and persists in products after processing. In humans as well as in animals, DON has been shown to induce both alimentary and hematological toxicities. Granulo-monocytic...

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In vitro cytotoxic effects of 4,4′-bipyridyl on normal human keratinocytes

M. Michel-Buono, J.-P. Buono, G. Serre, D. Dumont, more

Cell Biology and Toxicology > 1997 > 13 > 3 > 193-204

Recent epidemiological studies have brought to light a possible link between premalignant or neoplastic skin lesions (Bowen disease, squamous carcinoma) and occupational exposure to 4,4′-bipyridyl (4,4′B), a precursor in the synthesis of paraquat herbicide. The present study used a serum-free cell culture of normal human keratinocytes (NHK) and two skin-equivalent models to test the effects of exposure...

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Release of RANTES from nasal and bronchial epithelial cells

G.B. Altman, L.C. Altman, D.L. Luchtel, A.J. Jabbour, more

Cell Biology and Toxicology > 1997 > 13 > 3 > 205-213

RANTES is a chemokine with eosinophil attractant and activating activities. This study was undertaken to determine whether primary cultures of human nasal and primate bronchial epithelial cells produce RANTES and the effect of various cytokines and dexamethasone on the release of this chemokine. Nasal epithelial cells from 32 patients (HNE) and bronchial epithelial cells from 17 Macaca nemestrina...

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Applications of primary human hepatocytes in the evaluation of pharmacokinetic drug-drug interactions: Evaluation of model drugs terfenadine and rifampin

A.P. Li, M. Jurima-Romet

Cell Biology and Toxicology > 1997 > 13 > 4-5 > 365-374

The utility of primary human hepatocytes in the evaluation of drug-drug interactions is being investigated in our laboratories. Our initial approach was to investigate whether drug-drug interactions observed in humans in vivo could be reproduced in vitro using human hepatocytes. Two model drugs were studied: terfenadine and rifampin, representing compounds subjected to drug-drug interactions via inhibitory...

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DNA ploidy and autophagic protein degradation as determinants of hepatocellular growth and survival

P.O. Seglen

Cell Biology and Toxicology > 1997 > 13 > 4-5 > 301-315

Hepatocytes have the ability to go through specialized cell cycles, which, during normal developmental liver growth, result in the formation of binuclear and polyploid cells. In the adult rat liver, the majority of the hepatocytes (about 70%) are tetraploid, 15-20% are octoploid, and only 10-15% are diploid (about 50% in humans). One-third of the hepatocytes in either rats or humans are binuclear...

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Apoptosis in the liver and its role in hepatocarcinogenesis

R. Schulte-Hermann, W. Bursch, A. Löw-Baselli, A. Wagner, more

Cell Biology and Toxicology > 1997 > 13 > 4-5 > 339-348

Apoptosis seems to be the predominant type of active cell death in the liver (type I), while in other tissues cells may die via biochemically and morphologically different pathways (type II, type III). Active cell death is under the control of growth factors and death signals. In the liver, endogenous factors, such as transforming growth factor β1 (TGF-β1), activin A, CD95 ligand, and tumor necrosis...

article

The coculture: A system for studying the regulation of liver differentiation/proliferation activity and its control

A. Corlu, G. Ilyin, S. Cariou, I. Lamy, more

Cell Biology and Toxicology > 1997 > 13 > 4-5 > 235-242

article

Isolated hepatocytes - past, present and future

M.N. Berry, A.R. Grivell, M.B. Grivell, J.W. Phillips

Cell Biology and Toxicology > 1997 > 13 > 4-5 > 223-233

The first technique for large-scale preparation of isolated hepatocytes was described in 1953 and involved perfusion of rat liver under pressure with a Ca2+-free solution containing a chelating agent. Various modifications of this technique were in use over the next ten years, until it was demonstrated that cells prepared in this manner were grossly damaged, losing most of their cytoplasmic enzymes...

INFONA - science communication portal

Cell Biology and Toxicology

International Congress on Hepatocytes, Abstracts

In vitro effects of Celiptium and MR 14504 on mature rat Leydig cell testosterone production

Furaltadone cytotoxicity on three cell lines in the presence or absence of DMSO: Comparison with furazolidone

Comparison of amitriptyline metabolism in hepatocytes from streptozocin-induced diabetic rats and from non-diabetic rats

Comparative evaluation of the antiproliferative effect of cyclosporin A and γ-interferon on normal and HPV-transformed keratinocytes by cell counting, MTT assay and tritiated thymidine incorporation

Limitations of alginate gels as a culture model for the study of the effects of UVA radiation on human dermal fibroblasts

Establishment and characterization of an abrin-resistant cell line

Culture and characterization of juvenile rabbit tenocytes

L-Oxothiazolidine 4-carboxylate pretreatment of isolated human peripheral blood lymphocytes reduces sulfur mustard cytotoxicity

Synthetic hispidin, a PKC inhibitor, is more cytotoxic toward cancer cells than normal cells in vitro

In vitro modulation of the P450 activities of hamster and human lung slices

Structure-activity relationship in the induction of chromosomal aberrations and spindle disturbances in Chinese hamster epithelial liver cells by regioisomeric phenanthrene quinones

In vitro toxicity induced by deoxynivalenol (DON) on human and rat granulomonocytic progenitors

In vitro cytotoxic effects of 4,4′-bipyridyl on normal human keratinocytes

Release of RANTES from nasal and bronchial epithelial cells

Applications of primary human hepatocytes in the evaluation of pharmacokinetic drug-drug interactions: Evaluation of model drugs terfenadine and rifampin

DNA ploidy and autophagic protein degradation as determinants of hepatocellular growth and survival

Apoptosis in the liver and its role in hepatocarcinogenesis

The coculture: A system for studying the regulation of liver differentiation/proliferation activity and its control

Isolated hepatocytes - past, present and future

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