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Summary. Callus induction, somatic embryogenesis and plant regeneration were obtained in two cultivars of Sorghum bicolor (L.) Moench. Transverse thin celllayers from roots/epicotyls of 15dayold seedlings or of regenerated plantlets were used. Callus response depended on the genotype, the size of transverse thin cell layers, the level at which transverse thin cell layers were excised on the epicotyl,...
Abstract. Mature deembryonated cotyledons with intact proximal end of Vigna unguiculata were cultured on B5 basal medium containing varying concentrations of BAP. Thirtysix percent of the explants produced shoots on B5 medium supplemented with 8 x 10 6 M BAP. Cotyledon explants were preincubated for 24 h, inoculated with A. tumefaciens pUCD2614 carrying pUCD2340, cocultivated for 48 h and transferred...
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