Coconut acid oil (CAO) is a byproduct from the processing of coconut oil. CAO has a tremendous potential in the preparation of alternative fuel as biodiesel. In the present research investigation, CAO has been utilized for the preparation of biodiesel using Lipase AY Amano 30 (Candida rugosa) and Novozyme 40013 (Candida antarctica) in the presence of methanol using hexane as solvent. Initially, Lipase AY Amano 30 has been used for the biohydrolysis of neutral glycerides present in CAO, under the influence of water at 400C. Henceforth, the hydrolysed CAO was bioesterified with methanol in the presence of non-specific immobilized enzyme NS 40013 for the production of alternative fuel. The characteristics of CAO biodiesel (CAOB) was compared with diesel fuel and good outcome has been revealed. So cheap raw materials like CAO has been selectively utilized for the production of alternative fuel through a novel technology which may be useful for the partial replacement of present non-renewable fuels.
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