We report here the protocol for the preparation of 2-amino-N'-(1-(aryl-2-oxo-2H-chromen-3-yl)ethylidene)benzohydrazides compounds in moderate to excellent yields (46–86%) within a short reaction time (10–15 min), through a clean and efficient procedure. Additionally, the method has a wide substrate scope and provides an accessible route for the large-scale direct synthesis of 2-amino-N'-(1-(aryl-2-oxo-2H-chromen-3-yl)ethylidene)benzohydrazides. The products were assayed for their in vitro biological assay antimicrobial evaluation against two Gram-negative bacteria Escherichia coli MTCC 442, Pseudomonas aeruginosa MTCC 441 and antifungal activity against Candida albicans MTCC 227, Aspergillus Niger MTCC 282, Aspergillus clavatus MTCC 1323 at different concentrations which compared with ampicillin, chloramphenicol, ciprofloxacin, norfloxacin, nystatin, and greseofulvin in as standard drugs which are presented.
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