The main aim of the present study is to develop an automatic method to identify and classify the different cell types of bacilli bacterial cells in digital microscopic cell images using active contour method. Snakes, or active contours, are used widely in computer vision and machine learning applications, particularly to locate object boundaries. GLCM, HOG and LBP features are used to identify the arrangement of bacilli bacterial cells, namely, bacillus, cocobacilli, diplobacilli, palisades and streptobacilli using SVM classifier. The current methods rely on the subjective reading of profiles by a human expert based on the various manual staining methods. In this paper, it is proposed a method for bacilli bacterial cell classification by segmenting digital bacterial cell images using active contour model and extracting GLCM, HOG and LBP features. The experimental results proves that, the SVM classifier has yielded an overall accuracy of 97.2% with GLCM features, HOG features has yielded an accuracy of 74.8% and LBP features yielded 91.2% accuracy. The GLCM features with SVM classifier has got good classification results compared to HOG and LBP feature sets for bacilli bacteria cell types.
 Sigal Trattner and Greenspan, Automatic Identification of Bacterial Types Using Statistical Imaging methods, IEEE Transactions on Medical Imaging, Vol. 23(7), 807-820 (2004)
 Nicholas, B., Åke, H., Johan, W., Rocio, C-H. and Peter, K.B. (1998). Rapid determination of bacterial abundance, Biovolume, Morphology, and Growth by Neural Network-based Image Analysis. Applied and Environmental Microbiology, Vol. 64, No. 9, pp. 3246-3255
 Perner P. (2001) Classification of HEp-2 Cells Using Fluorescent Image Analysis and Data Mining. In: Crespo J., Maojo V., Martin F. (eds) Medical Data Analysis. ISMDA 2001. Lecture Notes in Computer Science, vol 2199. Springer, Berlin, Heidelberg
Financed by the National Centre for Research and Development under grant No. SP/I/1/77065/10 by the strategic scientific research and experimental development program:
SYNAT - “Interdisciplinary System for Interactive Scientific and Scientific-Technical Information”.