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A photothermal experimental system for intracellular thermal mapping is presented, which makes the use of aqueous CdTe/CdS/ZnS quantum dots as the thermal sensors and allows the measurement of temperatures in living cells embedded with the QDs via temperature-dependent photoluminescence (PL). Measured data show that the QDs synthesized in our laboratory have a thermal-PL sensitivity of 0.16nm//spl...
Computational electromagnetics modeling is presented for the design of multilayered nanoparticles for maximized forward light scattering. The design computation makes use of a recursive solution algorithm for multiscattering of light waves by multilayered core-shell nanoparticle aggregates. The algorithm is based on the analytical solution of Maxwell equations using the multipole-expansion method...
The purpose of our study was to evaluate the value of whole-lesion apparent diffusion coefficient (ADC) histogram parameters in distinguishing cervical cancers with different differentiation. A cohort of 18 patients (mean age, 53 years) with biopsy confirmed cervical cancers were involved in this prospective study. All of them underwent 3-T diffusion weighted imaging (DWI) with b values of 0 and 800...
Subpixel edge detection is critical in three-dimension computer-assisted intra-operative navigation based on a marker-based matching technique, because it significantly influences the accuracy of guidance. In order to increase the accuracy, a gray moment based method is proposed to extract the accurate contours of the connected areas. Firstly, a border following algorithm is used to acquire a rough...
This study proposes a computed tomography This study proposes a computed tomography This study proposes a computed tomography (CT) guided percutaneous intervention system based on infrared localization, aiming to accurately place the needle through the skin and into the lesion with real-time visual guidance information, especially in paracentesis and thoracentesis.
Cells can be regarded as a complex network, which contains thousands of criss-cross signal pathways. The mechanical properties of a cell reflect the structure and composition of its cytoskeleton and are closely related to the cellular biological functions and physiological activities. In this study, we develop a dynamical model with cellular viscoelasticity properties as the system parameters to describe...
Specific molecular recognition between antibody and receptor on cell membrane plays a central role in biological research. As a main component in the renin-angiotensin system (RAS), angiotensin II type I receptor (AT1R) mediates the classical biological actions of angiotensin II (Ang II). In this study, we applied atomic force microscopy (AFM) to characterize and quantify binding interaction between...
Precise control and point-to-point actuation of opto-thermocapillary flow addressed (OFB) bubble microrobots are critical for applications in micromanipulation. Here, we have developed and demonstrated a hybrid closed-loop control system capable of automated point-to-point actuation of a bubble microrobot in linear trajectories, and semi-automated vision-based position updates of a bubble microrobot...
Extracellular matrix (ECM) plays a critical role in regulating cell behaviors such as proliferation, spreading, migration and differentiation. Here we investigate ECM micro-patterns as a factor of cell-ECM interactions. Different sizes of circular ECM islands were fabricated using the microcontact printing (μCP) technique as an array of cell attachment sites spreading over the substrates. We investigate...
This paper presents a microarray platform that allows high-throughput single cell capture in large microwells to support single-cell culture. This work will facilitate the study of cell heterogeneity as well as high efficient stimulation parameters (chemical and physical) screening. An U-shaped single-cell trap, in combination of two streamline focusing arms, is placed at the bottom of each microwell...
We report a feasible light-addressable electrodepostion approach to realize rapidly controllable fabrication of 3D hydrogel structure on an organic photoconductive material electrode surface. The fabrication process is carried out by using an optical system setup. A pattern produced by a visible-light projection system is projected onto the titanium oxide phthalocyanine (TiOPc) coated chip as a virtual...
This paper presented a method of adopting Pt/MWCNT nanowire as a vibrator which is used to increase the quality factors of the MWCNT. A platinum nanowire growth was induced via the EBID method from a carbon nanotube (CNT) emitter with a trimelyl cyclopentadienyl platinum (CpPtMe3) precursor. The MWCNT was driven by the electrostatic force on its nature frequency. Two groups of experiments were carried...
The nuclear radiation exposure in Fukushima in 2011 has aroused the publics’ anxiety on nuclear safety. The alpha particles in such radiation may induce cancer and other serious diseases once entering the human body, yet high-sensitivity detection of alpha radiation remains unavailable and technically challenging. Here, we report the development of an effective and reliable alpha radiation measurement...
The aims of this study were to evaluate the clinical feasibility of using an Electronic Portal Imaging Device (EPID) for patient position verification, and to analyze for the EPID's limiting factor in the use of patient setup verification. The investigation involves 15 patients with different carcinomas. All of them were initially positioned by using a three-point laser alignment method and received...
As a new technology platform, the microfluidic concentration gradient chip (CGC) has recently attracted a large amount of interest in the field of biomedical engineering such as drug screening, chemical synthesis, trend research, cell apoptosis etc. In this work, a 4-inlet, 36-outlet stereo networks microfluidic CGC was brought forward and fabricated by 3D printing technology. Numerical simulations...
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