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This presentation will provide an overview of surgical molecular navigation using fluorescence imaging to visualize key normal structures and diseased tissue in real time.
Using an optofluidic chip with an integrated nanopore, a mixture of nanobeads and influenza viruses were opto-electrically detected. Different types of nanoparticles can be distinguished by different fluorescence wavelengths and fluorescence correlation functions.
We use time-resolved sensing and sparsity-based dictionary learning to recover the locations and lifetimes of fluorescent tags hidden behind a turbid layer. We experimentally demonstrate non-invasive target classification via fluorescence lifetimes.
The spin state of the silicon-vacancy centre in diamond and its optical accessibility have so far remained elusive. We here evidence spin-tagged fluorescence through resonant optical access to the electronic spin ½ of the centre.
We demonstrate that temporal focusing microscopy with structured illumination provides super-resolution even if wavefront distortion within the sample results in stretching the point spread function of the microscope.
We demonstrate utilization of femtosecond-duration laser pulses for two-photon-absorption LIF imaging of atomic species in nonequilibrium plasmas. Femtosecond excitation enables improved signal-to-noise ratio, suppression of photolytic interferences, kilohertz-rate imaging, and potential for collisional quenching-free measurements.
We have developed a nonlinear spectral unmixing algorithm that separates fluorescence excitation-emission matrix of multiple fluorophores affected by the inner filter effect. We evaluate this technique on simulated data and demonstrate its superior performance experimentally for a mixture of fluorophores.
The fluorescence is experimentally controled with bandgap engineering of structured metal surface. Grating duty ratio is optimized as 3/4 and narrow emission spectra are obtained by coupling between Fabry-Pérot cavity and surface plasmon polariton modes.
Hydrophobic and oleophobic surfaces have been used to deliver molecules and nanoparticles in given 2D arrays with spatial control. Effectiveness in sensing and assembly processes is shown, reaching ultra low sensitivity (aM) and precise positioning of colloidal nanocrystals.
Novel meta-coaxial nanoantennas are studied numerically, fabricated and experimentally characterized. These antennas provide local field enhancements of 200–800, super-localized fields with spatial FWHM of ∼1nm, and wide spectral ranges with FWHM bandwidths greater than 900nm.
We demonstrate a novel device, based on wedge prisms, that enables simultaneous imaging and fluorescence microscopy of multiple color channels and is simpler, more user-friendly, and less expensive than current commercial devices. Applications include ratiometric calcium imaging and co-localization of multiple labels.
We use thermophoresis to accumulate and quantify biomolecules under a laser-induced temperature gradient. As biomolecules accumulate at the heated region, the concentration of the molecules can be determined based on the level of accumulation.
I will review our development and application of fluorescence lifetime imaging implemented in microscopy, tomography and endoscopy to provide molecular readouts across the scales from super-resolved microscopy through imaging of disease models to clinical applications.
We present a microfluidic cytometer that sorts mammalian or yeast cells by laser force deflection following real-time multibeam, multiparameter fluorescence measurements, including photobleaching, lifetime and expression level, of the intrinsic fluorophores within each cell.
Estimating bio-distribution of fluorophore in tissue is challenging. In this paper, we demonstrated that our depth perturbation method can accurately determine depth of fluorophore located in tissue as deep as 5.8 mm (in epi-illumination geometry) by phantom experiments.
Inflammatory responses to lung injuries are mediated through enhanced production of reactive oxygen species (ROS). We describe the use of activatable NIR fluorescent agents with high sensitivity to ROS to image the lung during conditions of high inflammation.
We report on position and density control of nitrogen-vacancy (NV) centres created in type Ib diamond using localised exposure from a helium ion microscope and subsequent annealing. Spatial control to <380 nm has been achieved.
We combined voltage-sensitive dye imaging (VSDi) with gradient-index (GRIN) rod lens to study neural functions in mice vibrissae system. Neural activities evoked in the thalamic barreloids by single whisker stimulation were visualized in vivo.
We present a plasmonic nanobiosensor offering superior analyte delivery efficiency. Our experiments with virus-like analytes show more than an order of magnitude faster response time, and seven orders-of-magnitude dynamic concentration range for 103–109 particles/mL.
We present the measurement of photobleaching spectra of fluorescent proteins with nonlinear Fourier-transform spectroscopy using ultrabroadband femtosecond pulses. Photobleaching of two-photon excited fluorescent molecules occurs through one-photon excited-state absorption.
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