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Persistent Organic Pollutants (POPs) and heavy metal are two kinds of important pollutants in marine environments. Individual toxicity of naphthalene (one of POPs) or cadmium (a heavy metal) has been examined and reported, yet the joint toxic effects of them haven't been studied. Since chemicals usually exist as mixtures at low level concentrations in real environment, joint toxic effects on living...
The chemical composition of solid wastes from Silybum marianum fruits (WSS) and the contents of total polyphenols, total flavonoids and antioxidant activity of the extracts from WSS with distilled water, ethanol (40%, 70% and 95%) and methanol was investigated. Chemical analysis indicated that WSS contained high amounts of crude protein (25.58%), calcium, potassium and magnesium. The content of plumbum,...
Experimental technology and the latest development of Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry(MALDI-TOF MS) were introduced and its research value was highlighted through combining its application examples in environmental science, such as identifying the biodenitrification bacteria in wastewater, identifying the proteins differentially expressed under cadmium...
The characteristics and application properties of feather keratin were reviewed and the mechanisms of keratin degradation were briefly discussed. The mechanisms of physical pressure, membrane potential and complex enzymes were introduced and three actions or processes of mechanical keratinolysis, sulphitolysis and proteolysis were discussed. The actions of sulphitolysis and proteolysis functioned...
Objective To study the technology of separating and purificating of polysaccharides from Portulaca oleracea L‥ Method The polysaccharide was obtained from Portulaca oleracea L. by hot water extraction followed by precipitation with ethanol and purified with DEAE cellulose column chromatography. The molecular weight and the composition of the polysaccharides were determined by high-performance gel-permeation...
Four water-soluble polysaccharides named as SBP-11, SBP-12, SBP-13 and SBP-14 were obtained from Scutellaria barbata by defatting, boiling water extraction, deproteinating, decoloration and DEAE-52 chromatography purificaton. The carbohydrate content of SBP-11, SBP-12, SBP-13 and SBP-14 were 90.24%, 88.56%, 86.42% and 85.23%, respectively. SBP11 with molecular weight (MW) 1.8×104 D was composed of...
In the last decade, the role of NO in plants has received much attention. Due to its multifunctional activities in plants, NO is now being considered as a new candidate plant hormone. However, it is not very clear that the physiological mechanism of exogenous NO alleviates salt effects in vegetable plants. Therefore, an experiment was conducted to study the effects of exogenous NO on physiological...
The purpose of this study is to further improve the accuracy of predicting winter wheat grain quality with remote sensing, to enhance the prediction mechanism and to meet the demand for winter wheat production. In order to predict grain protein content (GPC) in winter wheat using landsat TM images, The experiment was carried out in Jiangsu regions during 2007–2009 winter wheat growth season. Based...
Give chrome shavings an alkaline treatment using CaO and MgO together to extract the gelatin. Then protein polypeptide is extracted by hydrolyze the residue, which is left in the first step, with 1398 neutral proteinase. The best process condition is that when the usage of CaO is 3% (base on the mass of chrome shavings), MgO is 3%, H2O is 300%, hydrolysis temperature is 80°C, and the reaction time...
During poultry processing, abundant of waste feather is produced, which is valuable for its high protein content. After the pretreatment, the feather is hydrolyzed to be polypeptide under alkali conditions. The best process condition is that when the usage of NaOH is 12% (base on the mass of dry feather), the amount H2O is 8 times of the feather, hydrolysis temperature is 100°C, and the reaction time...
Object: Rapid purification of 20S proteasome from fresh swine liver by ultrafiltration and ion-exchange membrane. Methods: 20S proteasome was first precipitated with ammonium, then separated by ultrafiltration membrane with cut molecular weight of 300 KD and 1000 KD, and finally separated by ion-exchange membrane. The purified fraction was verified by specific proteasome inhibitor epoxomicin, SDS-PAGE...
The interaction of the complex[Cu2Cl4(C17H20Cl2N2)2] with bovine serum albumin (BSA) has been investigated by fluorescence spectroscopy and UV-absorption under simulative physiological conditions. The experimental results indicated that the quenching mechanism of fluorescence of BSA by complex was a static quenching process. The binding constants(Ka) and the number of binding sites(n) were calculated...
In this study, antioxidant peptides from scallop were prepared through enzymatic hydrolysis using scallop skirt as the material. Firstly, the trypsin was one of the hydrolysis of scallop skirt. The content of AN was the standard of choosing the suitable enzyme for the hydrolysis. Finally, the aim of the study is to search the proper condition of trypsin and prospect for the future work.
Objective: To investigate influence of administration of Shenxiao decoction on the expression of NADPH oxidase, renal function and structure in diabetic nephropathy rat. Method: Experimental diabetic nephropathy model was induced by nephrectomy combined with streptozotocin (STZ) injection in sprague-dawley rat. Shenxiao decoction and Irbesartan were orally administrated respectively for 16 weeks,...
Objective: To assess the bone protective effects and mechanism of Polygonatum Sibiricum Polysaccharide (PSP) in ovariectomized rat. Methods: 60 female SD rats were randomly separated into 6 groups:control group (n=10); ovariectomized group (OVX) (n=10); the OVX + E2 group(OVX + E2) (n=10); the high-dose OVX + PSP group (OVX + HZ) (n=10); the moderate-dose OVX + PSP group (OVX + MZ) (n=10); the low...
Using two pairs of specific primers, DNA fragments containing the promoter PpsbA of psbA gene and terminator TpsbA of psbA gene, were obtained by PCR from Beta vulgaris. L chloroplast genomic DNA. Sequence analyzing results showed that the functional domains such as −35 domain, −10 domain and the translation control sequence motif in the cloned promoters the highly conserved to Nicotiana tabacum....
The cDNA of DREB transcription factor named OjDREB1 was obtained from Lilyturf (Ophiopogon japonicus) under the drought stress of 12h by RT-PCR method. Sequence analysis showed that cDNA of OjDREB1 is 1779 bp long, the open reading frame is 1062bp.The predicted protein has 353 amino acids with an estimated molecular mass of 38.69 kDa and an isoelectric point of 6.46. The deduced primary structure...
Cloning of new NAC transcription factors from maize (Zea mays). The length of new ZmNAC cDNAs were cloned by RT-PCR, designing the degenerate primer based on the conserved domains of NAC transcription factor. The length of ZmNAC cDNA sequence was isolated from maize, and it contain 750bp more or less. The results showed that a new center sequence of ZmNAC transcription factor was cloned in maize.
The main aim of this article was to develop a sensitive method to detect trace protein in Chinese Materia Medica injections (CMMIs). PVDF membranes of 9 mm2 were kept in sample of 10 ml, and the membranes were washed with methanol to remove the stained color from CMMI. Then, the membranes were dyed with 0.25% Coomassie Brilliant Blue solution for at least 30 sec. After the dyed membrane was washed...
The aim was to develop an analysis system of determination of twenty amino acids by ninhydrin reaction with FIA, and to find behavior of twenty amino acids in the system. The FIA adopted a dual flow path system in that water and ninhydrin solution were used as carrier liquid and reagent flow respectively, an adjuster for temperature adjusted the reaction temperature. The results showed that detection...
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