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The GC-ECD was used in this study. The optimization of preparation was performed by U9(94)uniform design method, With the rate of 2, 6-DNT-degrading result as investigative indexes, the influences of of ratio the concentration of substrate and PH, The speed of shaker and Temperature the result were screened by Uniform Design to found the optimum condition of degrading. Result the optimum condition...
Tetherin cDNA was amplified from total RNA prepared from HeLa cells by RT-PCR, then cloned into the eukaryotic expression vector pEGFP-C3. The generated pEGFP-Tetherin construct was transfected into COS-1 to determine the expression and the subcellular localization of GFP-Tetherin fusion protein. Under CLSM the localization of GFP-Tetherin fusion protein distributed outside the nucleus and co-localized...
In order to solve the increasing water environmental problems caused by NPSP, we selected Ashi River Basin of Acheng section as a research subject and study it's characteristics of NPSP. The results showed that the primary pollutants were TN and TP, and the primary pollution source was crop farming and stockbreeding. Then we put forward effective measures for prevention and control of NPSP.
Nowadays wood-based panels are widely used in furniture manufacture and interior decoration. They would release formaldehyde during the service for most of them being bonded with the formaldehyde-based adhesive. The formaldehyde does a great harm to people's health. Measurements should be taken to lower the formaldehyde emission. Several methods were introduced in this paper to solve the problem....
Coke oven gas is one of by-products generated in the process of dry distillation of coal to form coke. The coke oven gas is of high value in used as not only energy but also chemical material, but it contains hydrogen sulfide (H2S) gas which limits its application. Thus, it is necessary to remove H2S from the coke oven gas in order to use it as energy or chemical material. The thermodynamic analysis...
The adsorption of diesel oil in seawater on biodegradable adsorbing material and the influencing factors such as salinity, pH and temperature were studied. Results showed the adsorption of diesel fitted the second-order reaction model, and the saturated adsorption capability was 6.69g/g which can be reached in 10-20min. The diesel oil removal efficiency increased with the increment of salinity and...
To detect and identify of Enterohemorrhagic Escherichia coli O157:H7, the gene rfbE of O157:H7 was detected as the aim gene and the Agilent 2100 bioanalyzer was used as an analysis tool, which was compared with the conventional agarose gel electrophoresis. Finally, it was known that the rfbE gene was successful in detection of O157:H7. And the bioanalyzer was as 10 times sensitive as the agrouse gel...
Microbially assisted recovery of copper from low-grade chalcopyrite has been reported to be a very difficult process, conventional hydrometallurgical methods were limited by many parameters. This study focus on the design and the training of a Multi-Layer Perceptron classifier for the optimized preparation conditions for bioleaching of chalcopyrite. The proposed approach uses the heuristic Backpropagation...
The acute toxicity of herbicide atrazine to a species of green algae Scenedesmus obliquus, a species colorless heterotrophic flagellate Bodo saltans and two species of ciliates Tetrahymena priformis and Oxytricha saprobia was examined in laboratory tests. The results showed that the acute toxicity of atrazine to S. obliquus, B. saltans, T. priformis and C. pyrenoidosa was high. The median effective...
This paper proposed a hydrodynamic model for the Meiliang lake based on the Environmental Fluid Dynamical Code (EFDC). Considered the different speed of wind and the process of reservoirs operation, we have been analyzed the growth of microcystis aeruginosa. The results showed that: (1) when the wind speed is 3m/s, the velocity of water flow is more suitable for the growth of microcystis aeruginosa;...
Anaerobic biological technology has been widely applied in the treatment of low-concentration organic water recently. However, there are no successful engineering cases of using anaerobic reactors to treat municipal wastewater because of its low efficiency. This paper puts forward the technological process of using unequal spaced opposite floded plate Reactor to treat municipal wastewater. Through...
Colostrum contains higher concentrations of immune-enhancing nutrients and components than mature milk and its bioactivity has received widespread attention. In the present study, the protein fractions of bovine colostrum whey were isolated and purified by membrane filtration, series of chromatography on DEAE52 ion exchange column, gel filtration column and Econo-pac Q column. Four kinds of dominant...
High F- in water can be removed by Ca2+ in form of precipitation of CaF2 in water when their ion activity production(IAP) reaches up to equilibrium dissolution constant of fluorite based on the precipitation theory of insoluble minerals. Self-designed tank is used to generate a large number of Ca2+ and CO32- from dissolution of limestone under the effect of electric field, then the Ca2+ and F- movement...
A magnetically separated fly ash micro-sphere (MSFM) were prepared by citrate sol-gel process.The microstructure of the products were tested by SEM and EDS. It was found that the MSFM was coated with network of barium ferrite, which exhibited the magnetic absorption property. Then the TiO2 film photocatalysts immobilized on MSFM were prepared by sol-gel method, methods. The crystal phase and morphology...
The water quality characteristics of Jinan Queshan reservoir (which derived from Yellow River) are low turbidity and algal bloom. Based on those characteristics, the flofilter technology with flotation and filtration was developed, which was conducted to dispose of the eutrophic water with the algal bloom from Queshan Reservoir. The tested result stated that the removing rate of total algae was 72...
Organic compounds are the important pollution compounds in landfill leachates. For determinating organic compounds in landfill leachates, a pretreatment method for the analysis of organic components is the important step. In this paper, the landfill leachates were extract with liquid-liquid extraction (LLE) and solid-phase microextraction (SPE), respectively. The effect of different extraction methods...
To discuss the problem of excess sludge reduction, ozonation was used to treat sludge from aerotank.we studied influences to effluent COD, NH3-N and TP by excess sludge reduction. Test indicated that specific desaturase activity of excess sludge decreased with oxidation time; in mixture of sludge and water after oxidation, SCOD increased with time extending; the removal rate of COD and TP had no obviously...
To study the acute toxicity and the mutagenicity of Eriobotrya japonica extract. The oral acute toxicity test in mice , Ames test , micronucleus test in bone marrow and sperm abnormality test were carried out. The results shown that tolerated dose of 20g/kg body weight in mice could be regarded as virtually non-toxic. The micronucleus rates and sperm abnormality rates in all doses were not significantly...
The photocatalytic oxidation of methyl orange (MO) on commercial TiO2 nano-particles could be successfully enhanced by simple mechanical mixing with Fe3+ modified ZSM-5 zeolites (FeZSM-5). When a mixture of TiO2 and FeZSM-5 (TiO2:FeZSM-5 ratio=1:5) was added into the solution, methyl orange removal rate was 58.9% after 30 min of irradiation, which increased 22% compared with using TiO2 alone. However,...
In order to reveal the molecular mechanisms and the metabolic pathway of producing bioflocculant, the changes in protein complexes of F2 grown in minimal media versus bioflocculant media and those of F6 grown in minimal media versus bioflocculant media were respectively researched by SDS-PAGE. SDS-PAGE results indicated that the relative band volumes of three bands were obviously decreased in F2,...
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