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Intraoperative temperature measurement using magnetic resonance imaging has shown a great value as a noninvasive method. T2 relaxation time is one of the magnetic resonance imaging (MRI) parameters, theoretically, it is related to temperatures and signal amplitudess. in this paper we tried to find the relationship between temperature and porcine liver's T2 relaxation time by using CPMG sequence under...
This project focuses on the improvement of NDV production technology and innovation of the NDV vaccine preparation process with cell culture technology. Methods: To use primary chick embryo cell culture system in preparation of NDV anti-tumor vaccine. Results:In the new vaccine development process, the focused on maintaining and improving NDV titer while reducing the residual bovine serum. So that...
Gas chromatography-mass spectrometry (GC-MS) was used to study the compounds from ether extract of Catharsius molossus L., the traditional Chinese medicine, 'dung beetle'. Over twenty kinds of compounds from the ether extract were detected, and fifteen compounds whose match index was more than 800 were identified. All the identified compounds belonged to fatty acids, and the extract was rich in unsaturated...
Endocrine disrupters (EDCs) have caused great concerns for the safety of water environment and drinking water in recent years. This paper uses lab-cultured human breast cancer cells (MCF-7) to examine the changes of water estrogenicity during the drinking water treatment process and uses chemical analysis as supplementary information. The results showed that MCF-7 were extremely sensitive to the aqueous...
Soybean saponins have a lot of physiological activities and pharmacological action, and can be used to resist cancer, adjust immunity, prevent and treat cardiovascular diseases, and so on. However, the activities of soybean saponins in natural form are not the best. In this thesis, biotransformation of group B soybean saponins was studied to increase physiological activity of soybean saponins. The...
We developed a quantitative detection method that can reflect the degree of visceral cell oxidative DNA damage induced by exogenous compound exposures using 8-OHdG as a biomarker in vitro. In this study formaldehyde (FA) was used as a model exogenous compound and malondialdehyde (MDA) was used as a reference indicator. Rat liver cell suspension was applied in this in vitro exposure experiments as...
Chemoprevention including the use of natural products is always used as one of the main treatments for advanced cancer, and has been under intense investigation. In the present study, we investigated the inhibitory effect and mechanism of acetylshikonin on murine sarcoma 180 cells. The results showed acetylshikonin could inhibit the proliferation of sarcoma 180 cells and had obvious dose-effect relationship...
Photosensitization of quantum dots was activated with a LED light source (410nm) delivered at 5mw/cm2 to give a different dose by altering the radiation period. Cellular proliferative activity and apoptosis was measured using the cell counting kit-8 (CCK-8) assay. After four hours following photodynamic treatment, the degree of apoptosis was significantly increased, and the best experimental parameters...
The present study processed the absorption spectrum of CdSe nanocrystals (Ncs) which was modified by mercaptoacetic acid (MA). Based on this, the LEDs whose emission peak is near the absorption peak of the NCs were selected to form a LED array. The leukemia cell line HL60 was cultured in two 96-well plates and incubated with different concentrations of MA-Coated CdSe NCs. One of the 96-well microplates...
A new four-arm spiral antenna for microwave hyperthermia is presented in this paper. The finite element method was used to simulate the distributions of the electromagnetic fields and the specific absorption rate (SAR) of the four-arm spiral antenna in phantom muscle tissues. The simulation results show heating region of the four-arm spiral antenna can change as the frequency of input excitation....
In order to investigate the effects of electric pulses on cancer cells, HepG2 and L02 were exposed to electric pulses (1000V/cm, 100μs, 1Hz) for different time (8s, 15s, 30s, 60s). Annexin-V-FITC Kit and Flow cytometry were used to study the apoptosis of treated cells. The results showed that the electric pulses of 1000V/cm, 100μs, 1Hz for 8s couldn't induce tumor cells apoptosis. Apoptosis was observed...
Water-soluble low-molecular-weight chitosan(LCTS) was prepared by hydrogen peroxide oxidation method and their quaternized derivatives(QLCTS) was also synthesized using previous methods. Their antitumor activity was studied, and the results showed that the LMW and its quaternized derivatives inhibited the growth of pancreatic carcinoma cell strain PANC-1 in vitro, and the inhibitory rate reached 59...
The purpose of this article was to observe interaction between drug and cancer cell with fluorescence probes and molecular imaging technology. In order to study the effect of cell growth and apoptosis morphology of Human gastric adenocarcinoma SGC-7901 induced by n-butanol extract from Capparis spionosa L. (CSBE), laser scanning microscopy was employed to observe apoptosis imaging. In addition, the...
The classification of breast masses into benign and malignant categories plays an important role in the area of computer-aided diagnosis (CAD) of breast cancer. In this paper, one novel scheme based on multi-view information fusion is proposed, in order to improve the accuracy and the robustness of the classification and reduce the false positive rates. Five contour and shape features of the masses...
Ovarian Carcinoma (OvCa) is the most lethal type of gynecological cancer. The studies show that about 90% patients could be saved if they are treated in the early stage. In this study, a novel biomarker selection approach is proposed which combines singular value decomposition (SVD) and Monte Carlo strategy to early OvCa detection. Other than supervised classification methods or differential expression...
The purpose of the current study was to investigate the changes of serum proteome and to discover potential biomarkers from a publicly available proteomic ovarian dataset. A workflow that combines stationary wavelet transform with naive Bayes classifier was presented to select candidate biomarkers form 253 proteomic serum profiles of cancer and control. The method identified correlative mass points...
Nrf-2(Nuclear factor erythroid 2-related factor 2)/HO-1(Heme Oxygenase-1) signaling pathway plays an important role in the suppression of carcinoma, The study aims to investigate the effect of Curcumin on the expression of HO-1 and Nrf-2, which will provide a therapeutic intervention for treatment of carcinoma. SHSY5Y cells were treated with Curcumin at 0, 1.25, 5.0, 20.0 μM for the time course assay...
Objective To discuss the clinical diagnosis value of Multislice helical CT in renal cell carcinoma (RCC) and angiomyolipomas (AML). Methods 93 patients (78 were RCC and 15 were AML by pathologic diagnosis through postoperative examination of specimen) were retrospectively analyzed. CT number in ROI (region of interest) of these masses was recorded, CT number in ROW (region of whole mass) of these...
The purpose of this article was to investigate the apoptosis effect of the n-butanol extract from Capparis spinosa L. (CSBE) on human Gastric Cancer cells SGC-7901. MTT assay was used to measure the inhibitory rate of CSBE Morphological changes of apoptosis were observed with fluorescence microscope. Flow cytometry was employed to analyse the apoptotic rate of CSBE on SGC-7901 cells. Laser confocal...
Copy-number variation (CNV) constitutes a large proportion of total genomic variation and is increasingly recognized to be an extremely important risk factor for cancer. To examine the role of CNVs in glioblastoma, a genome-wide association study of CNVs in glioblastoma was conducted by assaying 221 tumor tissues and 28 normal tissues samples from primary glioblastoma multiform patients in TCGA project...
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