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Filamentous fungal strains were isolated from the leaves and fruits of Camellia oleifera with the anthracnose disease. The pathogenecity was tested following Koch's postulates. Our analyses identified that all 18 isolated strains were pathogenic to Camellia oleifera. The molecular analysis of the internal transcribed spacer regions of the most virulent isolate confirmed that it belonged to Colletotrichum...
Biochip plays an important role in biotechnology and many scientists also pay a lot of attention on this product. Thus, in this study we used MEMS and sacrificial layer techniques to manufacture the microfilter. It only depends on different development properties of the positive and negative photoresists. The feature size of filtration can be precisely controlled by the thickness of sacrificial layer...
A new type of effective coagulant Microbial Flocculants was prepared from A- spergillus oryzae and used as coagulant in the treatment of papermaking wastewater. The experimental results showed that it had a good coagulation effect in the treatment of papermaking wastewater in a wide range of pH value. The removal rate of SS and CODCr was 97% , 81 % respectively in the condition where temperature was...
The acute toxicity of herbicide atrazine to a species of green algae Scenedesmus obliquus, a species colorless heterotrophic flagellate Bodo saltans and two species of ciliates Tetrahymena priformis and Oxytricha saprobia was examined in laboratory tests. The results showed that the acute toxicity of atrazine to S. obliquus, B. saltans, T. priformis and C. pyrenoidosa was high. The median effective...
Selection the stuffing and optimization its surface can increase the biological treatment system capacity and buffer capacity. Biological deodorizations of hydrogen sulfide (H2S) was conducted by using modified ceramic which was made under different density of modifying agent as carrier in biotrickling filter. All modified ceramic had strong abilities to immobilize deodorization bacteria and the biofilm...
A newly isolated moderate thermophilic strain 1-7 producing alkaline and thermostable lipase previously was identified as Acinetobacter calcoaceticus, which was isolated from the soil collected from bay of Bohai in China. The lipase showed maximum activity at pH 9.0 and high thermostability. The lipase from Acinetobacter calcoaceticus 1-7 was purified by ammonium sulfate precipitation, ion exchange...
Hydrogenase is the key terminal enzyme in electron delivery within hydrogen metabolism in bacteria, which has been attracted many attentions. In the present study, crude enzyme was firstly prepared by cell broken. Then a kind of hydrogenase from Ethanoligenens harbinense YUAN-3 was purified by chromatography methods on Sephadex G-100 and DEAE 52 columns. The enzyme was purified 169-fold with 7.8%...
It receives much concern whether the transgenic foods, especially the antibiotic marker genes, are safe for human health all the time. In this paper, the transgenic Malus domestica Borkh. cv. Royal Gala carrying exogenous neomycin phosphotransferase (nptII) was used to study the npt II gene activity in transgenic apple fruits. The result showed that the npt II activities were detected in the most...
China is a great agricultural country. Reasonable development and utilization of rural energy is related to Peasant's human production and life and improvement of rural eco-environment. Because lack of energy is serious in recent years. China strengthened study on biogas technology and got rich findings. But there is relatively backward in extended application of biogas technology. Therefore,this...
The mass balance of COD, N and P in the wastewater treatment process was studied respectively on a trial basis by a pilot-scale A2/O system. Under three kinds of operating conditions which with different temperature (14°C, 18°C, 21°C) and mixed liquor internal recycle ratio (100%, 200%, and 300%), the flow and distribution of COD, N and P in the system were tested and analyzed. The variation of these...
A bacterial strain Pseudomonas sp. Zjwp-14 was screened out from soil, and used for the biotransformation of DL-2-amino-Δ2-thiazoline-4-carbonic acid to L-cysteine. This strain was identified based on its morphological, physiological characterization, 16S rDNA sequence determination and phylogenetic analysis. The bioconversion process was optimized by examining some key factors including cell cultivation...
Calculating methods for inhibitor concentration (IC50) leading to 50% activity lost (for diphenolase activity) of a novel parabolic competitive inhibitor (the trifluoromethyl-containing 1,2,3-triazole, TF-TA) and a novel mixed inhibitor ([E]-4-(1-imidazoylmethyl) cinnamic acid (ozagrel) were derived on the basis of kinetics of tyrosinase inhibition. These methods were applied to calculate IC50 values...
Dyes are natural compounds that make the world more beautiful through coloured substances. However, the release of coloured wastewaters represents a serious environmental problem and a public health concern. Colour removal, especially from textile wastewaters, has been a big challenge over the last decades, and up to now there is no single and economically attractive treatment that can effectively...
According to the characteristics of contaminated surface water, a stain of Micrococcus sp. (Micrococcus sp. N3-9P) that could remove CODCr efficiently was picked out from the slowly flowing oil-polluted riverbed sludge in Liaohe Oil Field, and then it was immobilized by chemical embedding technology. Through the conditional tests of immobilizing parameters, an improved co-immobilization technique...
With sewage plant aeration tank activated sludge as mixed bacteria source, Nitrobenzene concentration gradient of the chlorine used to increase the aerobic bacterial degradation of oscillating bottle method of domestication and enrichment culture. Separation and purification out of one can right-chloronitrobenzene as the sole carbon, nitrogen and energy of bacteria. Through physiological and biochemical...
Aqueous extraction and alkaline (NaOH) hydrolysis method were comparatively studied in polysaccharides extraction from submerged-cultured Agaricus blazei mycelia. The results show that the polysaccharide content and the total extract rate from alkaline extraction method were obviously higher than those from the aqueous extraction method. Optimization results by the orthogonal matrix method on polysaccharide...
The study evaluated the sorption of Zn(II) ions from aqueous solution by two low-cost materials, waste somatic and dead tea fungus. The rate and extent of accumulation were affected by pH, contact time and initial zinc ion concentration. The initial removal was rapid and equilibrium was established in less than 30 min. Good correlation coefficients were obtained when the pseudo-second-order kinetic...
Soil pollution is a worldwide problem. Both inorganic and persistent organic pollutants (POPs) possess high toxicity and long-term persistence. Biosurfactant based remediation technologies for contaminated soil are of increasing importance recently, and they have been demonstrated to be effective in uptaking both heavy metals such as Cd, Pb, Zn, Cu, and organic pollutants like hydrophobic organic...
In this study, artificial antigens for Bensulfuron-methyl (BSM) were prepared by means of two-step procedure of glutaraldehyde method. The hapten BSM was covalently attached to ovalbumin to synthesis immunogen (BSM-OVA), and hapten Benzyl sulfonamide (BS) was linked to BSA to prepare coating antigen (BS-BSA). The conjugates were identified by UV spectrophotometry and SDS-PAGE. After immunized with...
The filter media dimensions are the key design parameter in filter system. In this study, the ceramic pellets with diameter of 3-5 mm, 6-9 mm and 10-13 mm were employed in the vermifilters of D1, D2 and D3, respectively, for excess sludge treatment. The aim of this study was to optimize the diameter of filter media based on the stabilization degree of excess sludge and the corresponding physiological...
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