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The extravascular matrix is believed to carry negative charge due to its glycosaminoglycans (GAGs) composition. In the present study, we developed an electrodiffusion-convection model to investigate the mechanisms by which this negative charge affects the charged molecule transport through the interstitial space. The model predictions demonstrate that the apparent tissue diffusion coefficient of negatively...
TNFalpha stimulates SPHK in the monocyte, which leads to the expression of adhesion molecules on the cell surface. The adhesion of leukocytes to the endothelium is one of the early stages of the onset of atherosclerosis. In this paper, we have delineated the TNFalpha-induced and SPHK-dependent signaling pathway. In addition, we have developed a biomathematical model to qualify the SPHK time-dependent...
Because of the overwhelming scattering of light in biological tissues, the spatial resolution and imaging depth of conventional fluorescent imaging is unsatisfactory. Therefore, we present a dual modality imaging technique by combining fluorescence imaging with high-resolution noninvasive photoacoustic tomography (PAT) for the study of an animal tumor model. PAT provides high-resolution structural...
We present a strategy of bioinformatics analysis to exploit the current data available both on genomic and metabolic levels and integrate these at novel levels of understanding of metabolic disorders. PathAligner is applied to predict biomedical data based on a given disorder. A case study on urea cycle disorders is demonstrated. Both genetic data and metabolic information are retrieved. We integratively...
This paper introduced a straightforward and effective chromatographic data pre-processing method developing for utilization prior to chemometric analysis of large metabonomic dataset arising from high performance liquid chromatography. Nucleotides chromatographic fingerprinting in human urines was employed to validate the proposed method. Performance for discrimination cancer samples from healthy...
A set of protein pairs predicted to be interacting with high ratio of true positive is valuable for target selection in experiments like protein structure determination. Our goal in this paper is to investigate the problem of finding such a set of protein pairs in an organism by machine learning methods. Yeast genome was taken for this study and support vector machine was adopted as the classification...
To compare large genomic DNA sequences of related organisms and of different species, researchers need efficient methods to align many long sequences. We developed a new tool super multiple genome alignment (SMGA for short), specially for rapid multiple global alignment of genomic sequences. SMGA can align a data set consisting of 50M of 9 enterobacterial genomic sequences in 40 minutes. Our method...
A pocket blood sugar apparatus tested by enzyme electrode, which was designed using carbon and silver plasma as conducting materials. Both the work and reference electrodes are applied to the parts of enzyme electrode. The glucose oxidase is taken as the medium of blood sugar measuring. And the range of measuring glucose is about 50mg/dL - 500mgl/dL. It has better linear for the results and fit coefficient...
A novel disposable lactate biosensor was reported, which can measure the lactate in whole blood and other bio-fluids rapidly, accurately, expediently and directly. The biosensor was based on two kinds of electron mediator, the ferricyanide and the modified carbon nanotubes (CNT). Lactate oxidase (LOD) was coated on the electrodes. The sensor was fabricated by screen-printed techniques, the sample...
The carbon nanotube modified biosensors for respectively monitoring uric acid (UA) and total cholesterol in blood were studied. The transducers were based on two screen-printed carbon electrodes, a carbon working electrode and a reference electrode. For UA sensors, uricase was immobilized on the surface of electrodes together with potassium ferrocyanide as electron transfer mediator. For cholesterol...
Capacitive affinity sensors have shown great promise for disposable biosensors but have poor sensitivity as a direct sensor if the analyte molecule is small. In this paper, we report an indirect capacitive affinity sensor with silica nanoparticle tags to enhance the sensitivity for sandwich type of immunoassays. In these experiments, the anti-body-antigen-antibody complex (as in the standard indirect...
Haemodynamic changes related to activation of the human visual cortex were studied using optical imaging. The change in oxyhaemoglobin concentration in the visual cortex was estimated using a perturbation Monte Carlo (pMC) method. Comparison to a topographic map obtained using the modified Beer-Lambert law and interpolation is given
A CW type handheld near infrared tissue oximeter called P-Scan tissue imager was developed for real time imaging of tissue oxygen saturation ([StO2]) and hemoglobin concentration ([Hbt]). The probe consists of eight dual-wavelength light sources (690 nm and 830 nm) and eight photon detectors forming a 2.5 cm times 2.5 cm matrix. The local tissue oxygen saturation and hemoglobin concentration was calculated...
In this paper, a simulation model of glucose-insulin metabolism for Type 1 diabetes patients is presented. The proposed system is based on the combination of compartmental models (CMs) and artificial neural networks (NNs). This model aims at the development of an accurate system, in order to assist Type 1 diabetes patients to handle their blood glucose profile and recognize dangerous metabolic states...
Cryopreservation of human embryonic stem cells is an important and unsolved problem. A computer-controlled programmable cooler is used in the preservation of ES cells. Several effects have been experimentally studied, which include the cooling rate, the seeding temperature , the terminative temperature before the sample is plunged into liquid nitrogen. It is found that the constitution of cryoprotective...
The self-assembling of DNA and cationic polymers are of interest for their biological applications. In this paper, the interaction of DNA with PAMAM was studied by agarose gel electrophoresis, size and zeta-potential measurements and circular dichroism (CD) spectroscopy. DNA was shown to form stable complexes with PAMAM by gel electrophoresis. The mean particle size of the complexes was in the range...
Nano-structured four branched, blocked star vector (BSV) was molecularly designed by iniferter-based photo-living radical copolymerization method of N,N-dimethylaminopropylacrylamide and then N,N-dimethylacrylamide from four dithiocarbamate-derivatized benzene for a novel gene delivery non-viral vector. The conjugated pDNA complexes were satisfactorily stable in the condition of the electrophoresis...
When conjugated to CdSe/ZnS nanocrystals (quantum dots), the nucleobase adenine and the neurotransmitter dopamine quench fluorescence emission from in a manner strongly dependent upon the size of the quantum dot. The degree of quenching serves to predict the efficiency with which the conjugates are able to enter living cells. Along with quenching, the presence of specific receptors on the cells is...
Nanostructures of lysozyme molecules and lysozyme-nanogold conjugates were generated by atomic force microscopy in contact-, tapping- and force-distance-mode on mica in aqueous solution. In contact mode at high ionic strength, adjusted lysozyme concentration and lower loading force a monolayer of defined structure and orientation of lysozyme can be formed by the scan process of the tip. A lateral...
There are great challenges of developing high-throughput analytic tools in the post-genomic era. The microarray technology, as one of the most powerful high-throughput tools, has a lot of opportunities in meeting increasing enormous demands in the discovery of the molecular mechanisms of life systems. A number of the new concept microarrays will be developed and used in fundamental and medical researches...
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