The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
To compare large genomic DNA sequences of related organisms and of different species, researchers need efficient methods to align many long sequences. We developed a new tool super multiple genome alignment (SMGA for short), specially for rapid multiple global alignment of genomic sequences. SMGA can align a data set consisting of 50M of 9 enterobacterial genomic sequences in 40 minutes. Our method...
When conjugated to CdSe/ZnS nanocrystals (quantum dots), the nucleobase adenine and the neurotransmitter dopamine quench fluorescence emission from in a manner strongly dependent upon the size of the quantum dot. The degree of quenching serves to predict the efficiency with which the conjugates are able to enter living cells. Along with quenching, the presence of specific receptors on the cells is...
There are great challenges of developing high-throughput analytic tools in the post-genomic era. The microarray technology, as one of the most powerful high-throughput tools, has a lot of opportunities in meeting increasing enormous demands in the discovery of the molecular mechanisms of life systems. A number of the new concept microarrays will be developed and used in fundamental and medical researches...
This paper describes the detection of vesicular stomatitis virus using a novel capacitive immunosensor technology, whereby the respective antibodies for the antigens were used as a means for chemical detection on separate sensors. Devices were fabricated using standard etching and metal plating techniques, followed by immobilization of antibodies. Detection of antigen was performed by measuring voltage...
Here some progresses of core-shell nanoparticles (NPs) for biomedical applications have been reported. A new synthesis mechanism for preparation of core-shell NPs by using water-in-oil (W/O) microemulsion technique has been investigated. And the applications of the core-shell NPs on cell recognition, oligonucleotide detection, single bacterial detection, pH nano-sensor for single cell, DNA recovery...
A genetic differential amplifier is made using the control elements of genes. The output mRNA level is proportional to the difference between the concentrations of two input proteins. The active element is engineered from the right operator of bacteriophage lambda. Mutations are introduced to yield the correct gain characteristic and to provide a bias level; the latter allows for the representation...
Viral envelopes can be used as an effective vehicle to deliver imaging tracers as well as therapeutic drugs and genes. However, the current methods for in vivo tracking of viral envelopes are limited. This purpose of this study is to investigate dynamically the in vivo biodistribution of viral envelopes using positron emission tomography (PET) imaging. The hemagglutinating virus of Japan envelope...
The identification of protein-protein interactions along with their spatial and temporal localization is vital data for assigning functional information to proteins. Historically, these data sets obtained from fluorescence microscopy, have been analyzed manually, a process that is both time consuming and tedious. The development of an automated system that can measure the location dynamics of the...
This paper presents a method to estimate accurately the parameters involved in the HIV-I infection. An application to the evaluation of the drugs effectiveness and therapeutical failures is presented
Oligonucleotide fingerprinting of rRNA genes (OFRG) is a method for identifying arrayed ribosomal RNA genes (rDNA) through a series of hybridization experiments with short oligonucleotide probes. Due to its low cost and high speed, it is an effective tool for analyzing microbial communities. OFRG relies on probe sets that can discriminate large collections of clones. Although the currently used probe...
Automated chip-based technologies for clinical diagnosis may have great facilities in the area of life science and medicine. This paper presents the lab-on-a-chip design for the assay of HIV, which includes the sample preparation, reaction, and signal amplification module. A laser induced fluorescence system is also designed for real-time monitor of the signals
The investigation of interactions between two kinds of monoclonal antibodies and SARS virus with a label-free protein array technique were presented in this paper. The performance consists of three parts: a surface modification for ligand immobilization/surface, a protein array fabrication with an integrated microfluidic system for patterning, packaging and liquid handling, and a protein array reader...
Nanometer-sized particles such as luminescent quantum dots (QDs) and energy-transfer nanoparticles have unique optical, electronic, and structural properties (e.g., signal brightness, photostability, and multicolor light emission) that are not available from traditional organic dyes and fluorescent proteins. Here we report the use of color-coded nanoparticles and dual-color fluorescence correlation...
The aim of this work was to develop a HIV-1 control strategy based on the nonlinear geometric control theory. Such a strategy was applied on a three variables deterministic model that describes the characteristic stages of the HIV-1 infection. The control design developed in this work allows the exact linearisation of the model. The results show that the nonlinear control developed is effective to...
We have developed an array of sensors for the oral pathogen Streptococcus mutans (S. mutans) using an enzyme-linked immunosorbent assay (ELISA) on a polydimethylsiloxane (PDMS) device. The model bacterial analyte, S. mutans, has been implicated in the initiation and progression of dental caries. The PDMS was modified with 3-aminopropyltriethoxysilane (APTS) and glutaraldehyde to covalently crosslink...
To compare large numbers of genomic sequences of related virus, such as HIV, biologists have an increasing need for a method that can efficiently handle hundreds, even thousands, of genomic sequences accurately enough to correctly align these conserved features. In this paper, we introduce a new and efficient tool named SMA that can easily accommodate large-scale virus genomic sequences. A high-throughput...
Economic considerations make the conventional chest radiograph (X-ray) film an important ingredient in the diagnostic process. An initial clinical investigation for patients with suspected lung ailments is the study of the chest X-rays. The problem of detection for diseases in their early stages are well known using X-ray. A technique involving wavelets coefficient as the feature vector and Andrew's...
Conventional strategies of gene therapy using viral vectors result in suboptimal localization and potentially dangerous distal spread of vector. We hypothesized that site-specific delivery of adenoviral gene vectors could be achieved from a polyurethane (PU) film through a mechanism involving anti-viral antibody tethering. PU films were formulated with a collagen coating. Anti-adenoviral monoclonal...
Nonlinear properties of biological suspensions have been previously presented as a bulk phenomenon, however, some authors consider that they are generated at the electrode-electrolyte interface and are reflected as a bulk property phenomenon. They were mainly ascribed to the H+-ATPase present in the plasma membrane of these cells. In this paper, we describe the construction of a dual-cell nonlinear...
There is an urgent need for tools to unravel the complex interactions and functionalities of genes. As such, there has been much interest in reverse-engineering genetic regulatory networks from time series gene expression data. We use an artificial neural network to model the dynamics of complicated gene networks and to learn their parameters. The positive and negative regulations of genes are defined...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.