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In order to investigate the mechanism of the recognition of antibiotics with biomembrane, thermodynamic properties of the phosphoinositides (PIs) interacting with aminoglycoside antibiotics are studied by use of isothermal titration calorimetry. The results suggest different groups in the antibiotics have varied affinity with the PIs and neomycin B has higher affinity with phosphoinositides. PI binding...
Adsorption and retention on soil particles are vital behaviors affecting the amount of allelochemicals in soil, which perhaps results in their accumulation. To investigate the accumulation mechanism of three phenolic acids i.e. p-hydroxybenzoic acid, benzoic acid and cinnamic acid, which existed in the soil of poplar (Populus Sect. Aigeiros Duby) plantation, we studied their sorption and retention...
17 tomato cultivars inoculated with larva nematodes were used in this paper to investigate their resistance to root knot nenatodes. The results showed that gall index of LA1312, 3601, HA4402, LA1007 were higher than other tomato cultivars. Compared with control ‘Caihong101’, root activity of LA0655 was higher, and the lowest root activity was observed in 3601, whose gall index was the highest among...
BST-2 is a new kind of antivirus protein that is induced by IFN α/β. The BST-2 gene fragment was amplified by RT-PCR from pig kidney cells (PK-15) and subcloned into the pLenti7.3/V5-TOPO vector and the positive clone was filtrated. The positive plasmids were transfected into the HEK293T cells. The expressed protein was analyzed by SDS-PAGE. Vesicular stomatitis virus (VSV)-CEF restrain experiment...
Electroless plating of Ni-Fe alloy on poly (ethylene terephthalate) (PET) fabrics was investigated. Scanning electron microscopy (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) were used to characterize morphology, structure, composition of the PET fabric coated with Ni-Fe. The effects of plating parameters on the deposition rate, surface morphology and the composition of deposits...
Electrocatalytic oxidation of indomethacin on a modified multiplewalled carbon nanotubes (MWCNTs) cathode in aqueous solution was studied in this paper. The experiments were carried out to explore the effects of initial concentration, electrolytic voltage, pH, concentration of electrolyte and electrolysis time on removal rate of indomethacin. The results of show that the removal rate reached 78% by...
Ethidium Bromide (EB) is the most common dye in DNA gelelectrophoresis, but it is also a strong carcinogen and has strong mutagenicity which are harmful to experimenters' health and environment. In order to avoid the hazards and improve the safety of the experimenters, the safer dye should be used to replace EB. In this study, the staining results of different nucleic acid dyes, namely EB, Greenview...
This study was planned to determine the amount of input energy, output energy, energy-use efficiency and energy productivity for different tillage treatments. Energy equivalent was used in the study to converting energy input. Trial results showed that there were significant differences (P<0.05) among the three treatments in terms of energy indices and for crop yield. It was found that fertilizers...
An engineered Streptomyces lydicus strain with 6-phosphofructokinase gene (pfkA1) over-expression was constructed by recombinant DNA technology. Biomass, pH value, glucose and streptolydigin concentrations were measured and the fermentation kinetics were calculated. The cell growth and glucose consumption of the engineered strain was faster in the early period, and then slow down in the later stage...
This experiment use Sthenoteuthis oualaniensis egg as material through appropriate pre-processes to make the contained cholesterol released completely, then by using subcritical 1,1,1,2-tetrafluoroethane (R134a) extraction technology with orthogonal experiment to determine the best technical conditions for the removal of cholesterol from Sthenoteuthis oualaniensis egg powder. The results showed that...
Porous magnetic chitosan microspheres were prepared with reversed-phase suspension methodology using epichlorohydrin as cross-linking reagent and using CaCO3 as porogen for adsorption copper (II). Scanning electron microscopy (SEM) and Transmission electron microscopy (TEM) confirmed that the microsphere was homogeneous and highly porous. Experiments were performed at different pH of solution, contact...
The author researches on the allelopathy effects of different viscosity (0 ∼ 2.5g.mL−1) C. Cathayensis exocarp extracting solution, on green bean, wheat, soy bean and corn seeds. By calculation of the pot plants morphological and physiological indexes, the result shows: the viscosity (0.1∼0.5g.mL−1) solution could greatly promote the seed germination. The effects of the viscosity (0.1g.mL−1) solution...
AFLP technique was used to elucidate systematic relationships among 26 cultivars of Tree Peony. Six AFLP primer combinations (M+3 and P+3) were used to produce recognizable 757 bands, among which 623 bands are polymorphic. The percentage of polymorphic bands was 82%, suggesting abundant genetic diversity among sect. Moutan. Clustering analysis showed that some cultivars of dwarf and tall peony types...
Inappropriate thrombus formation in blood vessels causes cardiovascular disease (myocardial infarction, stroke, pulmonary embolism and so on) which is the most common cause of mortality at present. Fxa is an important factor in blood coagulation cascade. It is an effective and safe method to treat blood coagulation diseases by inhibiting FXa to control blood coagulation. In this paper, a new anticoagulant...
Herein, we demonstrate a new method for parallel depositing CdS nanowires (NWs) on the large-scale substrate with a high deposition yield. The method is based on blade-coating. First, CdS NWs solution is dripped onto the substrate surface. Second, before the CdS NWs solution evaporates, the blade slides along the substrate surface to assemble the CdS nanowires. The mechanism involving ordered alignment...
Power generation in microbial fuel cell (MFC) at different cathode electrode surface area was examined in two-chambered system with sodium acetate as carbon sources. When the anode surface area of 66 cm2 was used, the maximum power density increased by 44% when the cathode surface areas were increased from 66 cm2 to 336 cm2 and decreased by 21% when the cathode surface area was reduced to 14 cm2,...
By adding zinc chloride to the surplus sludge and using the traditional method for making activated carbon, we prepared the activated carbon adsorption deodorant and studied the function, benefit and security properties of the adsorption deodorant. The results show that the deodorant under the activation temperature of 600°C has more mesopore, its iodine adsorption value is higher than 300mg/g. Wastewater...
We develop a simple method for the parallel assembly of nanowires (NWs) based on oriented polymer nanofiber films. The nanowires dispersed in solution are aligned at the central space of parallel nanochannels formed by the well-oriented nanofibers as a result of the capillarity. Then, a general contact printing is used to realize the transfer of the nanowires from the donor nanofiber film to a receiver...
A novel domestic wastewater treatment system for reuse was proposed, which consisted of a microaerobic EGSB reactor unit and the following chemical phosphorus removal unit, as a low-cost and highly efficient process. In order to confirm the feasibility of the domestic wastewater treating technique at ambient temperature for reuse through the combination of the micro-aerobic EGSB reactor and the chemical...
Glutamate decarboxylase (GAD) is the key enzyme for the production of gamma-aminobutyric acid (GABA), a multifunctional agent which biosynthesis has attracted intense interest. Though immobilized GAD favors the preparation of GABA in several ways, its efficiency and enzyme activity recovery are damaged by the separation of purification and immobilization of enzyme. In order to simplified the process...
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