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Ethidium Bromide (EB) is the most common dye in DNA gelelectrophoresis, but it is also a strong carcinogen and has strong mutagenicity which are harmful to experimenters' health and environment. In order to avoid the hazards and improve the safety of the experimenters, the safer dye should be used to replace EB. In this study, the staining results of different nucleic acid dyes, namely EB, Greenview...
AFLP technique was used to elucidate systematic relationships among 26 cultivars of Tree Peony. Six AFLP primer combinations (M+3 and P+3) were used to produce recognizable 757 bands, among which 623 bands are polymorphic. The percentage of polymorphic bands was 82%, suggesting abundant genetic diversity among sect. Moutan. Clustering analysis showed that some cultivars of dwarf and tall peony types...
Using two pairs of specific primers, DNA fragments containing the promoter PpsbA of psbA gene and terminator TpsbA of psbA gene, were obtained by PCR from Beta vulgaris. L chloroplast genomic DNA. Sequence analyzing results showed that the functional domains such as −35 domain, −10 domain and the translation control sequence motif in the cloned promoters the highly conserved to Nicotiana tabacum....
The cDNA of DREB transcription factor named OjDREB1 was obtained from Lilyturf (Ophiopogon japonicus) under the drought stress of 12h by RT-PCR method. Sequence analysis showed that cDNA of OjDREB1 is 1779 bp long, the open reading frame is 1062bp.The predicted protein has 353 amino acids with an estimated molecular mass of 38.69 kDa and an isoelectric point of 6.46. The deduced primary structure...
To provide microbial evidence for technique improvement of anaerobic baffled reactor (ABR) - integrated oxidation ditch - biological aerated filter (BAF) combined processes for landfill leachate treatment, microbial community structure of the treatment system under different recycle ratios were investigated by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) combined with...
Cloning of new NAC transcription factors from maize (Zea mays). The length of new ZmNAC cDNAs were cloned by RT-PCR, designing the degenerate primer based on the conserved domains of NAC transcription factor. The length of ZmNAC cDNA sequence was isolated from maize, and it contain 750bp more or less. The results showed that a new center sequence of ZmNAC transcription factor was cloned in maize.
Cyanobacterial blooms were thought to be initiated by increased availability of phosphorus (P), alkaline phosphatase(AP) play a crucial role in P metabolism. Anabaena FACHB709, a species closely relative to cyanobacterium Anabaena flos-aquae has well-established genetic manipulation system, we have chosen this organism as a model to characterize four genes predicted to encode alkaline phosphatases...
Transformation of disease-resistant gene NPR1 to Watermelon was studied by ovary injection pathway after artificial pollination. Transgenic plants were obtained. Studies showed the injection of DNA solution at about 24 hours after pollination resulted in the highest transformation rate. PCR and southern blotting hybridization results showed that NPR1 gene were transformed into Watermelon.
The germinating capacity of wheat was investigated in the presence of butyhted hydroxy toluene (BHT) with ultraviolet (UV) radiation. The influence of UV and BHT on the growth of wheat was evaluated by means of variability of chromosome of germinating root tip cell mitosis and activity of superoxide distamuse (SOD) of malt. The results show that the germinating capacity of wheat reaches 87% when the...
To investigate the regulation of Ets family transcription factors Ets1 and Ets2 on the development of mouse testis and elucidate the effects of Ets1 and Ets2 on self-renewal and differentiation of spermatogonial stem cells. Mouse testis tissues were collected from specific developmental stages including the postnatal days 1, 5, 10, 15, 20, 25, 30, 35, 40, 50 and 70; busulfan peritoneal injection was...
In this study, the antigenic site B/C of S gene of transmissible gastroenteritis virus (TGEV) was cloned into prokaryotic expression vector pET-28a and the recombinant plasmid was transferred into E.coli BL21 (DE3). The fusion protein was expressed after induction with IPTG. The SDS-PAGE purified protein was used as immunogen. Six-week-old BALB/c mice were immunized three times with recombinant protein...
A total of 46 isolates of Shiga toxin-producing Escherichia coli (STEC), including 23 O157∶H7 and 23 non-O157 STEC strains, were analyzed for antimicrobial susceptibilities and the presence of class 1 integrons. All STEC strains were resistant to sulfamethoxazole trimethoprim, and the rate of resistance to ampicillin, and streptomycin was 30.4% and 28.3% respectively. Five isolates exhibited resistance...
According to the chicken's IL-7 gene sequences available in GenBank, a specific primer pairs was designed by Primer. To appraisement and sequencing, the total RNA extraction in local mucosal tissues of gastrointestinal and respiratory of chickens after administration probiotics, the products were amplification by the RT-PCR. The levels of IL-7 mRNA expression were analyzed by real-time fluorescence...
Selective liquid media with a serial of Pb2+ concentrations were used for screening lead-resistance microorganisms from sewage and industrial waste water, and one microbe strain lyh3A with strong Pb2+-resistance was gained. With Pb2+ concentration in medium increasing, it was tested the maximum tolerance to Pb2+ concentration of lyh3A was 1.5 g/L. This strain was identified as Aspergillus niger through...
Nearly 40 hereditary disorders in humans result from an increase in the number of copies of simple DNA repeats in genomic DNA. DNA repeats expansion mutations are dynamic and ongoing within tissues and across generations, leading to disease. These DNA repeats seem to be predisposed to expansion because they have unusual structural features, such as hairpins, slipped-strand DNA, DNA unwinding elements,...
The [Cu-C6H5ClOS- C5H7NO3 -1-10-phen]2+ interacted with the Calf thymus DNA in solution was studied by fluorescence - spectrometry. The model that the complex interacted with DNA was found: electrostatic action and intercalation.
This project utilize surgery combine chemical method to establish rat model of thromboangitis obliterans. To appraise the model we use the method of pathology, immunology and molecular biology. The result shows that injection with laurate sodium to Femoral Artery can successfully establish rat model of thromboangitis obliterans that similar to human natural disease. Rat model pathological changes...
A biomembrane that was used to make a biosensor for detecting inhibitor of ammoia oxidation was prepared by entrapping enriched ammonia-oxidizing bacteria (AOB) in polyvinyl alcohol (PVA). AOB were enriched by fed batch cultivation with using return activated sludge from A2/O process as seed. Enrichment of AOB was confirmed by denaturing gradient gel electrophoresis (DGGE) analysis. The optimum conditions...
DNA conformational change and breakage induced by Cr(VI) and curcumin in the present of H2O2 were characterized by the atomic force microscopy. Incubation of DNA with curcumin and Cr(VI) in the presence of H2O2 caused DNA double-strand breaks significantly. Cr(VI) and H2O2 also breaked mildly DNA double-strands. On the basis of these results, we think hydroxyl radicals and reactive oxygen species...
An inductive domestication method for removing SO2 from tail gas contained SO2 were set up. A group of desulfuration bacterium are obtained with this method domesticating the bacterium in oxidation ditch of city sewage treatment plant over 6d with low concentration SO2 gas. The desulfuration bacterium have an ability with biodegradation rate of 600 mg(SO32−)/(L.h) during 0.5h, and degradation efficiency...
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