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Environmental risk is ascribed to contamination level as well as the potential decontamination capability of the contaminated sites. In some cases, natural attenuation by biodegradation may dominate self cleanup as microorganisms always try to survive, which reduces risk to human health. Conventional assessment is based on sequential monitoring of concentrations, which requires a large number of samples...
The pulA gene enconding a thermostable pullulanase of 844 amino acids was cloned by PCR amplification from the genomic DNA of Thermotoga maritima MSB8. We successfully expressed the pulA gene in a protease-deficient strain Bacillus subtilis WB600, and the expressed pullulanase was secreted into the culture broth. Preliminary results showed that the pullulanase exhibited optimal activity at 90°C, and...
Gene expression analyses by probes of hybridization from mRNA to cDNA targets arrayed on membranes or activated glass surfaces have revolutionized the way of profiling mega level gene expression. The main remaining problems however are sensitivity of detection, reproducibility and data processing. During processing of microarray images, especially irregularities of spot position and shape could generate...
Cytochrome P450 4F3 (CYP4F3) catalyzes the inactivation of leukotriene B4 by ω-oxidation in human neutrophils. Here, we examined the mRNA and protein expression of the CYP4F3 in HL-60 cells, an acute promyelocytic leukemia cell line, in the presence of hydroquinone(HQ). We found hydroquinone led to a decrease in cell proliferation and a increase in cell apoptosis in a dose-dependent and time-dependent...
The activities in low temperatures of a mesophilic alkaline protease TC4 was improved by directed evolution. The process involved random mutagenesis by error-prone PCR and DNA shuffling followed by first screening on skim-milk plate and second screening by 96-well plate with AAPF as the substrate. It yielded a mutant T31, with a kcat 5 times and a catalytic efficiency kcat/Km 6.33 times greater than...
For molecular biological detection of Mycobacterium tuberculosis, PCR methods with primers targeting different regions specific for mycobacterium tuberculosis complex are used worldwide. However, some uncertainties exist concerning the specificity of certain target regions and the sensitivity. To identify the methods which are best suited for diagnostics, 9 single-round and 2 multiple PCR systems...
Ceratoides lanata is the native sand-fixing plant in deserts of America Continent. As an even-green undershrub with extremely strong antifreeze capability, Ceratoides lanata had been considered as the winterfat and introduced into many countries including China. To explore the mechanism of cold resistance and to clone genes directly involving the strong antifreeze capability, a normalized cold-stressed...
The ability to model complex regulatory networks is a big challenge in current biology research. Recently many computational methods have been developed to address this challenge. Compared with those methods using single data source, approaches integrating multiple data sources are expected to identify more reliable regulatory relations. Here, we present an easy but powerful method to integrate genome-wide...
MicroRNAs (miRNAs) are small noncoding RNAs which regulate gene expression. In this research, we used the Multiz alignments data of 44 Vertebrates to retrieve syntenic homolog sequences. And we identified 37 members of miR-1302 family among 71 potential homolog sequences. Then we analyzed the 37 homologs of hsa-mir-1302s by RepeatMasker and found all of these sequences are derived from MER53 elements...
Two strains named W7 and W16 were isolated by liquid enrichment culture from the corn field where atrazine has been chronically applied. Observed by gram stain and SEM, W7 belonged to gram-positive bacteria and the single cell was short bacilliform; W16 belonged to gram-negative bacteria and the single cell was spherical. Through physiological and identification and 16SrDNA sequence analysis, two...
It is well understood that the yak originates from China, but facts regarding origin time and evolutionary relationship with other species of Bovini, are largely disputed. Here, we have analyzed the phylogenetic relationship of eight representative species of Bovini, based on the Cytochrome b gene of mitochondrial DNA and TSPY gene of chromosome Y. We also estimated the divergence times of these different...
The partial sequence of the psbA gene from Cladosiphon okamuranus was amplified by PCR and their sequences were analysed. Neighbor-joining method was used to build phylogenetic trees according to the psbA sequence among 11 species, C. okamuranus included, that are affiliated to the algal order Ectocarpales. The results showed that the nucleotide sequence is 737 bp and the sequence shares high homology...
Based on distance distribution of nucleotides in a DNA sequence, a new distance measurement, average distance of four nucleotides, was proposed. The new index can be used to describe the regularity of four nucleotides distribution in a given DNA sequence. According to the new measurement, 17 complete mammal mitochondrial genomes have been analyzed and then cluster analysis and principal component...
Filamentous fungal strains were isolated from the leaves and fruits of Camellia oleifera with the anthracnose disease. The pathogenecity was tested following Koch's postulates. Our analyses identified that all 18 isolated strains were pathogenic to Camellia oleifera. The molecular analysis of the internal transcribed spacer regions of the most virulent isolate confirmed that it belonged to Colletotrichum...
The entomopathogenic fungus Beauveria bassiana, an important biological control agent of lepidopteron, is also the key pathogen to cause epizootic among the silkworm, Bombyx mori, a kind of insect very economically important as producer of silk in China. At present, very little is known about the isolates that are associated with the endemic or epizootic phases of this disease agent. Random amplified...
A bacterial strain Pseudomonas sp. Zjwp-14 was screened out from soil, and used for the biotransformation of DL-2-amino-Δ2-thiazoline-4-carbonic acid to L-cysteine. This strain was identified based on its morphological, physiological characterization, 16S rDNA sequence determination and phylogenetic analysis. The bioconversion process was optimized by examining some key factors including cell cultivation...
In the ABC model, MADs-box proteins play an important role in floral development. As one of the MADs-box proteins, PI behaves as B function organ-identity genes acting in the second and third whorls of the flower to control petal and stamen identity. To find genes involved in the floral transition of pineapple guava, we cloned the full length cDNA encoding a MADs-box protein in Coolidge pineapple...
REMAP (retrotransposon-microsatellite amplified polymorphism) is a kind of molecular markers based on retrotransposons. In this study, the genetic diversity of 73 eggplant varieties was examined by REMAP molecular marker. Results showed that 1992 bands were amplified with seven primers designed according to the sequences of MAGGY, BARE-1 and Sukkularc, the ratio of polymorphic bands was 100%, the...
Modification of histone proteins is associated with chromatin accessibility which profound epigenetic effect on DNA transcription, repair and replication. The SET domain proteins are recognized as regulating gene expression through lysine residues of histione methylation. Previous reports classified the SET domain proteins for 5 classes and 19 groups. In this study, we identified 15 barley SET domain...
Single-cell gel electrophoresis (SCGE) is a useful technical tool to detect DNA damages. In this work, tobacco plants (Nicotiana tabacum var. xanthi) were cultivated in different concentrations of nitrobenzene, and the DNA damages were analyzed by using SCGE. The results showed that the DNA damages increased with increasing nitrobenzene concentrations. Results of the present study suggest that nitrobenzene...
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