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The fluorescence quenching of a single molecule can be controlled using a plasmonic device made of a metallic or negative-index slab and a metallic nanoparticle. The concept of dark near-field fluorescence imaging is introduced.
Microscopic density characterization of transparent material with depth resolution is achieved with four-wave mixing microscopy. The density change inside a bent optical fiber with a curvature radius of 3.4 mm is successfully detected.
The fabrication of periodic poled Lithium Tantalate single crystal fibers by the laser heated pedestal growth method were achieved with in-situ monitoring of transient poling current. The whole cross section of the fiber is poled through as seen from the confocal measurement.
Raman microspectroscopy is used for the first time to study chemical and structural transformations in RPE cells exposed to cw and short-pulsed high-intensity light irradiation.
Novel schemes to optimize the focusing condition of Gaussian beam are proposed. Superresolution is achieved along a DOF 4 times larger than the confocal parameter. The experimental results are presented by use of an OALV.
We observe dramatic changes in the spectrum of radiation scattered from a near-field tip with decreasing tip-sample distance. This arises from a balance between direct tip scattering and signals associated with the near-field image dipole.
We present nanometer scale axial localization of fluorescent markers to probe subcellular structures using self-interference based fluorescence microscopy. We show probing the membrane topography of a gram-negative bacterium, Shigella flexneri, and discuss further applications.
The field of photoacoustic tomography has experienced considerable growth in the past few years. Although several commercially available pure optical imaging modalities, including confocal microscopy, two-photon microscopy, and optical coherence tomography, have been highly successful, none of these technologies can provide penetration beyond ~1 mm into scattering biological tissues because they are...
A nanometre scale, picoNewton force probe based on advanced optical tweezers control of transparent microstructures will be presented. The probe will be applied to dynamic bio-molecular interactions in solution
The potential of biomolecular microarrays on glass for high-throughput kinetics assays has not previously been fully exploited. We demonstrate real-time label-free optical detection of antibodies binding to drug-antigen microarrays using oblique-incidence reflectivity difference (OI-RD) microscopes.
In-line holographic microscopy is used to retrieve the 3D distribution of the refractive index perturbation, resulting from the nonlinear propagation of 35fs IR pulses in transparent media, including the Kerr effect and plasma strings.
We have demonstrated nondestructive 3D imaging of the femtosecond laser written 3D structures of both index change and optical breakdown within fused silica by using the optical coherence microscopy providing high lateral and depth resolution.
We present a new technique for imaging magnetic domains with a confocal optical resolution. It is based on a magneto-optical pump-probe imaging (MOPPI) using femtosecond laser pulses. Its efficiency on various magnetic structures is demonstrated.
We combined high resolution laser spectroscopy and microscopy to identify individual molecules in two independent microscopes. Then the Stark effect was exploited to tune the transition frequencies of the molecules and thus obtain indistinguishable single photons.
Multiple image planes in a multiphoton microscope are acquired simultaneously through novel photon counting electronics and previous scaling limitations are overcome by a new Yb:KGW femtosecond oscillator laser design.
The composition of fluids mixing inside of microchannels, as measured by CARS microscopy, is related to the flow field for the purpose of resolving non-symmetric velocity profiles.
With miniaturized tube lenses and two-dimensional asynchronous scanning of the microelectro-mechanical-system mirror, we demonstrated a 24 Hz frame-rate miniaturized two-photon fluorescence/second-harmonic-generation microscope system. Sub-micron transverse resolution of sectioning images can be achieved.
We demonstrate second-harmonic-generation (SHG) microscopic imaging on the polyhedral inclusion bodies (PIB) of nucleopolyhedrovirus in living cells. Due to a body-centeredcubic arrangement of polyhedrin trimers, these PIBs generate SHG with polarization anisotropy.
Using scanning near field optical microscopy (SNOM), we observed the focusing effect provided by negative refraction in a 2D photonic crystal. Experimental observations are analyzed in light of FDTD 3D simulations.
The contrast in nonlinear coherent microscopy is improved by using spatial phase shaping of the incident laser beams. This approach exhibits promising applications for biomedical imaging studies.
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