Most of applications based on cold cathode work in pulse driving mode. It is reported here that the field electron emission characteristics from carbon nanotube cold cathode by driving millisecond width pulse voltage is investigated. A peak emission current of 5.06 mA with the current density of 253 mA/cm2 is obtained from 0.02 cm2 carbon nanotubes film by driving 1 ms width with 10 Hz frequency pulse voltage, and it is about twice larger than the maximum current (∼2.5 mA) in continuous voltage. The relation between the emission current and pulsed width is also studied, showing that the peak emission current vary hardly with changing the pulse width with a constant frequency.