In this paper, we deal with the technique for detection of the location of markers on characteristic points for motion capture, and adopt the classic LED marker technique. The reason that we adopt this technique is to perform motion capture even in places where dark and light are mixed. For tracking the markers, most on-marker techniques have problems, such as difficulty in distinguishing between markers. Therefore, we proposed a new technique in which each marker is distinguished by means of the luminescence pattern of the LED according to the Walsh Function. By using this new technique, reciprocal interference between markers is eliminated and distinguishing between markers becomes easy. To evaluate the usefulness of the proposed technique, we performed computer simulation of the motion of eight markers and experimented using a machine in a case with four markers. As a result, we were able to derive and distinguish each marker's course within a certain distance.