Biofouling has become a major concern for many medical devices, which urges the development of effective antiadhesive materials. In this study, Pluronic F-127-dopamine (PF127-DA) was synthesized via coupling a catechol group to both ends of PF127. Cell adhesion assay showed that surfaces modified with PF127-DA outperformed those modified with poly (ethylene glycol) (PEG), PEG-DA, PF-127 or PF127-glutathione in both short-term and long-term applications. Different from antifouling materials with only one anchoring group in each molecule, PF127-DA molecule bears two catechol groups, and competently provides robust and efficient surface modification. Bifunctionalized PF127-DA shows promise in antifouling application in biomedical devices.