Repetitive, paired peripheral and transcranial stimulation targeting the cerebral cortex can increase cortical excitability, outlasting the stimulation period. It is unknown whether paired stimulation specifically targeting the spinal cord can modulate spinal excitability. We tested whether the H-reflex facilitation from a sub-threshold conditioning TMS pulse could modulate spinal excitability if delivered repetitively.In 13 healthy subjects, we delivered single-pulse TMS (80% RMT) for the right soleus muscle, 20ms prior to an electrical peripheral nerve stimulus delivered over the posterior tibial nerve on the same side at 0.1Hz during 15min.PNS alone evoked an H-reflex of 0.25mV±0.06 SEM, while pairing of TMS and PNS facilitated the H-reflex to 0.7±0.11mV. TMS–PNS pairs delivered at 0.1Hz for 15min progressively increased in the evoked response to ∼130% (r 2 =0.97) of the starting amplitude (normalized to 1stmin). Post-intervention, H-reflex threshold decreased (pre=12.9±1.7mA; post=11.6±1.6mA; p=0.04), as did the stimulus intensity at maximum H-reflex amplitude (pre=23.5±02.8mA; post=21.6±2.6mA; p=0.03), and recruitment curve width (pre=11.6±1.5mA; post=10.93±1.4mA; p=0.03). No such changes were observed with intervention of PNS or TMS alone.Paired stimulation targeting spinal facilitatory interactions, when applied repetitively, can increase spinal excitability during and after the intervention.Spinal associative stimulation may have potential for neuromodulation in spinal cord injury patients.