d- and l-glyceric acids are human metabolites responsible for two different diseases. Excess excretion of d-GA causes d-glyceric academia/acidurias, while excess excretion of l-GA causes hyperoxaluria type 2, PH II. Enantioselective, potentiometric membrane electrodes (EPMEs) based on maltodextrins I (dextrose equivalence (DE) 4.0–7.0), II (DE 13.0–17.0) and III (DE 16.5–19.5) as chiral selectors are proposed for the determination of l-glyceric (EPMEs based on maltodextrins I and III) and d-glyceric (EPME based on maltodextrin II) acid (l- and d-GA). EPMEs based on maltodextrins I and III can be reliably used for the analyses of l-GA using direct potentiometric method, in the concentration range of 10 −8 to 10 −6 and 10 −6 to 10 −3 mol/L, respectively, with very low detection limits (1.19×10 −9 and 1.0×10 −7 mol/L, respectively). The EPME based on maltodextrin II was successfully used for the enantioanalysis of d-GA in the 10 −5 to 10 −3 mol/L concentration range with detection limit of 1×10 −6 mol/L. The enantioselectivity of EPMEs was determined over l-glyceric acid (or d-glyceric acid), and their selectivity over creatine, creatinine and some inorganic cations such as Na + , K + and Ca 2+ . Simply polishing to obtain a fresh surface ready to be used in a new measurement can regenerate the surface of the electrodes.