The paper is devoted to investigation of the luminescent properties of Dy3+ and Dy3+-Ce3+ doped single crystalline films (SCF) grown by LPE method from PbO–B2O3 flux. We have found that the YAG:Dy and YAG:Dy,Ce SCFs possess bright cathodoluminescence in the visible range and good scintillation figure of merit. For this reason LPE grown YAG:Dy and YAG:Dy,Ce SCF are proposed for different applications, namely, as cathodoluminescence screens or screens for microimaging. The Dy3+ co-doping can be also proposed for improvement of the scintillation efficiency of the Ce3+ doped garnet compounds in the SCF form due to Dy3+→ Ce3+ energy transfer and removing the trap related centers in the above RT range.