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The small molecules detection remains a challenge for diagnostics. Due to their size, difficulties arise in finding selective and sensitive probes as well as developing appropriate detection format based on sandwich and/or signal amplification assays. In this study, we combine multiple strategies: a) the use of aptamer probes, short oligonucleotides strands selected in order to present strong selectivity...
In this paper, a home-made SPR analytical system and a denaturalized bovine serum albumin (dBSA) SPR chip were used to detect biotin based on the method of an inhibition immunoassay. The experimental was compared with a commercial protocol. The conditions of regeneration were optimized. The dBSA SPR chip was fabricated by a self-assembling method and the derivative of biotin was immobilized on the...
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