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TheKlebsiella pneumoniae ozenaeKIIIA strain was isolated from the River Rhine soon after a serious mercury pollution episode and was selected for mercury resistance as well as for intergeneric DNA mobilization helper potential. This transfer helper capacity was shown to be related to the presence of a Tn3-like transposable element,Tn5403.Because transposon-mediated fusion was found to be involved...
Two plasmids, pTF-FC2 and pTC-F14, that belong to the IncQ-like plasmid family were isolated from two related bacteria, Acidithiobacillus ferrooxidans and Acidithiobacillus caldus, respectively. The backbone regions of the two plasmids share a sufficiently high amount of homology to indicate that they must have originated from the same ancestral plasmid. Although some of their replication proteins...
Bacteria belonging to the Gram-positive actinomycete species, Rhodococcus erythropolis, are diverse not only in terms of metabolic potentials but the plasmids they encode. It was shown previously that the R. erythropolis AN12 genome harbors a 6.3kb cryptic plasmid called pAN12, which is a member of the pIJ101 family of plasmids. Here we show that pAN12 is conjugatively mobilizable into other rhodococcal...
The nucleotide sequence of a smallest cryptic plasmid pRK10 of Serratia marcescens ACE2 was determined. When compared to the all other plasmids reported so far from S. marcescens in sizes of over 70kb, pRK10 is only 4241bp long with 53% G+C content and has five coding sequences representing a coding percentage of 65.41. This small plasmid consists of one Tdh gene, four mobilization genes, mobCABD,...
Plasmids of IncQ-family are distinguished by having a unique strand-displacement mechanism of replication that is capable of functioning in a wide variety of bacterial hosts. In addition, these plasmids are highly mobilizable and therefore very promiscuous. Common features of the replicons have been used to identify IncQ-family plasmids in DNA sequence databases and in this way several unstudied plasmids...
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