The objective of this study is the extraction and concentration of ryanodol, an insecticidal compound contained in a Canarian endemic plant, Persea indica, using supercritical fluid antisolvent fractionation (SAF). The pesticide has been first extracted from P. indica using ethanol; then, the ethanolic solution has been concentrated using the SAF technique, in which supercritical CO 2 is used to precipitate selected, non soluble compounds. The quantification of ryanodol in the different extracts was accomplished using HPLC–MS. Operating at 15.0MPa and 35°C (flow rates of 2.38kg/h for CO 2 and of 5.7mL/min for the ethanol solution) a fraction with a ryanodol content of 37.7% w/w is obtained, starting from the liquid extract containing only 7.5% w/w of this compound.