Five new mexicanolide-type limonoids, carapanolides C–G (1–5), together with two new phragmalin-type limonoids, carapanolides H–I (6, 7), were isolated from the oil of Carapa guianasis AUBLET (Meliaceae) seeds. Their structures were elucidated on the basis of spectroscopic analyses using 1D and 2D NMR spectra and FABMS. Carapanolides C (1), E (3), and I (7) exhibited moderate activity in the P388 (IC 50 17.9μM in 1, 15.8μM in 3) and L1210 cell lines (IC 50 13.3μM in 1, 18.1μM in 3, 16.9μM in 7). On the other hand, Carapanolide D (2) exhibited a strong inhibitory effect in the HL-60 cell line (IC 50 11.0μM), Carapanolides F (4) showed inhibitory activity in the L1210 cell line (IC 50 15.9μM), and the cytotoxic activity of Carapanolides I (7) was moderate in all cell lines.