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The primary structure of two myoglobins (Mbs), isolated from the heart and muscle of Equus asinus L. and Struthio camelus L., respectively, was determined using a combined approach based on Edman degradation and mass spectrometry. The strategy allowed the determination of donkey Mb sequence, which was found to be identical to the horse Mb, as also confirmed by ESI/Q-TOF mass spectrometry. Indeed,...
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