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In this work, two proteins, Z-domains and bovine casein, were autodisplayed on the outer membrane of the same Escherichia coli cells by co-transformation of two different autodisplay vectors. On the basis of SDS-PAGE densitometry, Z-domains and bovine casein were expressed at 3.12×105 and 1.55×105 proteins/E. coli cell, respectively. The co-autodisplayed Z-domains had antibody-binding activity and...
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