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The antitumor sulfonylurea LY237868 (N-(4-aminophenyl-sulfonyl)-N -(4-chlorophenyl)urea) was conjugated through the A ring to α-cyclodextrin or agarose bead material (Affigel 10) to prepare impermeant conjugates for activity measurements and affinity isolation of binding proteins from serum. When conjugated to α-cyclodextrin, the resulting LY237868 conjugate inhibited both NADH oxidase activity and...
Plasma membrane vesicles isolated from HeLa cells grown in suspension culture contain a protein disulfide-thiol interchange (protein disulfide-like) activity. The activity was estimated from the restoration of activity to inactive (scrambled) pancreatic RNAase. RNAase activity was measured either by hydrolysis of cCMP or by a decrease in acid precipitable yeast RNA. The ability of plasma membrane...
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