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The serine protease inhibitor phenylmethylsulfonyl fluoride is shown to cause partial inhibition of bilitranslocase transport activity in rat liver plasma membrane vesicles. This condition can be fully reversed by means of pyridine-2-aldoxime methiodide, indicating that the carrier has undergone sulfonylation. Protection against inactivation is afforded by both bilirubin, the natural substrate, and...
Human erythrocytes, preincubated with different concentrations of calcium chloride (0.17-1.67 mM) showed hemolysis after addition of bilirubin (72 μM). Hemolysis was observed only when cells were incubated first with calcium followed by bilirubin and not vice versa. This hemolysis was found to be dependent upon several factors such as concentration of bilirubin, time of incubation of erythrocytes...
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