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We describe a dye–metal (polyhydroxybenzenesulfonephthalein-type dye and a transition metal) complex-based total protein determination method. The binding of the complex to protein causes a shift in the absorption maximum of the dye–metal complex from 450 to 660nm. The dye–metal complex has a reddish brown color that changes to green on binding to protein. The color produced from this reaction is...
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