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A nuclear magnetic resonance (NMR) method was implemented to assess in vivo oxygenation levels by a quantitative determination of the 1 H NMR myoglobin (Mb) resonances. The proximal His-F8 N δ H at 70–90ppm and Val-E11 γCH 3 resonance at –2.8ppm, reflecting deoxygenated (deoxy-Mb) and oxygenated (met-Mb) states, were alternately recorded. The method was developed in vitro choosing...
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