Procedural learning is crucial in everyday life; it underlies the acquisition of motor, cognitive, as well as social skills. Previous studies highlighted the supporting role of the right dorsolateral prefrontal cortex (DLPFC) in this process; however, the role of the left DLPFC remained controversial.In the present study we investigated the effect of left, right and sham anodal transcranial direct current stimulation (tDCS) of the DLPFC on a probabilistic sequence learning task which was performed by healthy young adults.We found that stimulation over the left hemisphere disrupted learning compared to both sham and right stimulation.This result is in line with previous findings highlighting an interhemishperic asymmetry in the role of the DLPFCs in probabilistic sequence learning.