To find new effective agents against alopecia, we established our original tissue culture system using 4-week-old C3H mice. At first we screened various kinds of synthesized compounds in our company using this culture system, and after that we examined the hair regrowth activity of the compounds selected by the first screening on hair clipped mice. Finally we chose KF19418 and KF15773 which have hair growth promoting activity equal to or higher than minoxidil. 10 μM of KF19418 promoted cell proliferation in hair matrix cell culture system four to six times as much as the control. This suggests that KF19418 directly targeted hair matrix cells. On the other hand, KF15773 did not show promotive effect on cell growth in this system. Thus, it is speculated that KF15773 targets other kinds of cells in the hair follicles.