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1,3-Diacylglycerol (1,3-DAG) production by esterification of fatty acids (FA) and glycerol in a packed bed bioreactor using a 1,3-regioselective immobilized lipase was studied. Esterification was effectively performed by circulating the reaction mixture between a packed bed column and a water removal vessel. The rate of 1,3-DAG synthesis was increased with increase in temperature up to 50 o ...
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