CD1c molecules sample lipids within the cell and present these lipid ligands at the cell surface. Once at the cell surface, CD1c restricted T cells review the CD1c-lipid complex in order to discriminate healthy from diseased cells. It has been previously shown that the CD1c of cancerous thymocytes present lipids that are recognized by cytotoxic T cells. Unfortunately, little is known pertaining to the lipid ligands that are bound and presented by the CD1c of a T cell lymphoma or leukemia.In order to understand the nature and breadth of intracellular lipid ligands presented by CD1c, a recombinant soluble CD1c was constructed, expressed in the SUP-T1 T lymphoma cell line, and the stable transfectants were cultured in a hollow-fiber bioreactor for sCD1c protein production. Soluble CD1c was affinity purified, lipid ligands were extracted from the purified CD1c protein, and the CD1c ligands were analyzed by mass spectroscopy.The data demonstrate that the CD1c of a T-cell lymphoma presents lipid ligands with six predominant head group moieties. In regards to diversity of the lipid moiety, we observed an array of radyl group linkages and chain lengths ranging 14-20 carbons long as well as various degrees of unsaturation.These data establish a lipid profile for the CD1c of a T cell lymphoma. Such data will now support immune assays that elucidate reactive CD1c cancer ligands, comparisons with ligands from other structurally distinct CD1 molecules, and comparisons to CD1c ligands from alternative cell lineages. Realizing the nature of biologically relevant CD1c ligands contribute to understanding the role of CD1 ligands in T cell initiated immunity.