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A derivatization reagent, 2-hydrazino-1-methylpyridine, was developed for the liquid chromatography–electrospray ionization-mass spectrometry (LC–ESI-MS) of oxosteroids. The reagent quantitatively reacted with oxosteroids at 60°C within 1h and the resulting derivatives of the mono-oxosteroids provided a 70–1600-fold higher sensitivity compared to intact steroids. However, HMP was unsuitable for di-oxosteroids,...
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