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Microbial lipases (E.C. 3.1.1.3) produced by Geotrichum sp. and Rhizopus sp. were prepared and tested as catalysts in the esterification of isoamyl alcohol and butyric acid in a solvent-free system. Response surface methodology (RSM) based on a five-level, two-variable central composite rotatable design (CCRD) was used to optimise this process, with respect to important reaction variables-substrate...
The culture medium including nitrogen source, carbon source and metal ions, for lipase from Penicillium camembertii Thom PG-3 was optimized and the optimal medium consisted of soybean meal (fat free) 4%, Jojoba oil 0.5%, (NH 4 ) 2 HPO 4 , 0.1% Tween 60, initial pH 6.4 and the inoculation was at 28 o C for 96h. The lipase activity produced was enhanced 3.9-fold and reached...
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