Dendrobium nobile Lindl. is a popular temperate Chinese orchid commonly marketed as a traditional medicinal plant. Seedlings of Dendrobium nobile Lindl. produced floral buds (33.3–34.8%) precociously on a defined basal medium (1/2 MS) containing paclobutrazol (PP 333 ) at 0.5mgL −1 or thidiazuron (TDZ) at 0.1mgL −1 within 4 months of culturing. The frequency of floral buds formation can be further increased to 95.6% by growing seedlings in a PN (PP 333 0.3mgL −1 +NAA 0.5mgL −1 )-containing medium followed by transfer onto 1/2 MS medium with PP 333 and TDZ (PP 333 +TDZ). However, flower developed was deformed under 25°C but it developed fully when grown in a lower temperature regime (23°C/18°C, light/dark) for 45 days. Under optimal condition, in vitro flowering was observed about 6 months after seed sowing.