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Addressing the need for validated tests for the study of Nervous Necrosis Virus (NNV, Betanodavirus) immunity, ELISA protocols were developed, optimized and compared for detection of serum antibodies against the virus in vaccinated fish. Competitive and indirect ELISA were performed using several combinations of immunoreagents, pursuing the highest specific reactivity. Reference sera were obtained...
An experiment was conducted to examine the potential for the complete replacement of fishmeal (FM) and fish oil (FO) in diets for barramundi, Lates calcarifer. A series of diets were formulated to the same digestible protein and energy specifications, but which were designed with FM inclusion levels at 300, 200, 100 or 0g/kg and FO at 100%, 30%, 15% or 0% of the added oil in the diets (4×4 factorial...
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