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A method for reactivation of inactivated horseradish peroxidase (HRP) with ethyleneurea and allantoin was studied and exploited in a continuous assay of hydrogen peroxide by micro-flow injection HRP-catalyzed luminol chemiluminescence. It is necessary to maintain the activity of immobilized HRP constant during the assay of hydrogen peroxide because the HRP is used repeatedly. If no reactivating reagents...
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