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A highly sensitive sensor for biochemical oxygen demand (BOD) has been developed. Using Pseudomonas putida instead of glucose and glutamic acid artificial wastewater (AWW) was employed for calibration of the BOD sensor. Typical response times of the BOD sensor were 2–15min, and the service life of the immobilized microorganisms is >10 days. The optimal BOD response was observed at 30°C and pH 7...
Biosensors for the selective determination of L-lactate and L-malate in wine based on robust solid composite transducers are presented. Transducers comprised a solid binding matrix having hydrophobic skeleton, e.g. 2-hexadecanone, graphite and NAD + . The enzymes, L-malate or L-lactate dehydrogenase and diaphorase, were placed onto the transducer surface and covered by a dialysis membrane,...
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