We report the allele and genotype frequencies in Italians of eight unlinked commonly utilized polymorphic loci: HUMTH01, HUMFES/FPS, HLA-DQA1, LDLR, GYPA, HBGG, D7S8 and GC. Genomic DNA from at least 100 individuals was amplified by PCR and typed after electrophoresis (HUMTH01, HUMFES/FPS), or reverse dot-blot (HLA-DQA1 and the other five loci). The allelic frequencies determined were compared with available population data. These results are useful for population and individual identification studies.