α-Amylase, a very critical enzyme for hydrolysis of starch into simple sugar and it has various applications in industrial settings. This study reports the identification of Bacillus sp. MB6 which produces increased amount of enzyme from less required resources. To optimize the yield of enzyme, we used various combinations of parameters. The most optimized conditions for production of amylase enzyme from the bacterium Bacillus sp. MB6 are pH of 6, temperature of 37°C, and incubation period of 48h. Condition of enzymatic activity were also examined and the results show that pH of 6, a temperature of 55°C, and a reaction time of 30min are the best available conditions for its activity. Purification of enzyme by 1.63 fold enhanced the specific activity of enzyme based upon its activity analysis as compared with unpurified enzyme. Enzyme kinetics studies show the Michaelis constant (Km) to be 5.45mg/ml and maximum velocity of the reaction (Vmax) to be 24.15mg/ml/min. In conclusion, we report enzyme production and purification methodology that exhibit better yield of alpha-amylase for commercial applications.