Large-scale, monodispersed titania microspheres have been synthesized by a facile hydrothermal method using FC-4 as surfactant at 150°C. The diameter of the microspheres can be conveniently tuned by changing the concentration of titanium precursor. The microsphere is composed of densely packed radially aligned rutile nanorods which are uniformly 16nm in diameter. The nanorods are grown along the rutile (110) crystal plane with a preferred orientation in the [001] direction. A mechanism for the formation of the microspheres has been proposed, in which the surfactant FC-4 plays important roles.